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Metabolic profile of follicular fluid in patients with ovarian endometriosis undergoing IVF: a pilot study.
- Source :
-
Reproductive biomedicine online [Reprod Biomed Online] 2024 Aug; Vol. 49 (2), pp. 103912. Date of Electronic Publication: 2024 Feb 24. - Publication Year :
- 2024
-
Abstract
- Research Question: What are the metabolic characteristics of follicular fluid in patients with ovarian endometriosis undergoing IVF?<br />Design: This was an exploratory cohort study on endometriosis. In total, 19 infertile patients with ovarian endometriosis diagnosed by laparoscopy, and 23 controls matched in terms of age and body mass index (women with infertility due to male or tubal factors) were enrolled in this study. All patients underwent IVF treatment with a gonadotrophin-releasing hormone antagonist protocol, and follicular fluid was collected at oocyte retrieval. The metabolomics of follicular fluid samples was analysed using an ultra-high-performance liquid chromatography Orbitrap Exploris mass spectrometer (UHPLC-OE-MS). The best combination of biomarkers was selected by performing stepwise logistic regression analysis with backward elimination.<br />Results: Fifteen metabolites were identified as biomarkers associated with endometriosis. A final model containing 8-hydroxy-2-deoxyguanosine, biotin, n-acetyl-L-methionine and n-methylnicotinamide was constructed. Receiver operating characteristic analysis confirmed the value of these parameters in diagnosing endometriosis, with sensitivity of 94.7% and specificity of 95.7%. Enrichment analysis via the Kyoto Encyclopedia of Genes and Genome showed that 15 metabolites were enriched in eight metabolic pathways.<br />Conclusion: Metabolomics based on UHPLC-OE-MS effectively characterized the metabolomics analysis of follicular fluid in patients with ovarian endometriosis. These findings may provide a new basis for better understanding of how diseases progress, and for the discovery of new biomarkers.<br /> (Copyright © 2024 Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.)
- Subjects :
- Humans
Female
Pilot Projects
Adult
Metabolomics
Biomarkers metabolism
Biomarkers analysis
Infertility, Female metabolism
Chromatography, High Pressure Liquid
Ovarian Diseases metabolism
Case-Control Studies
Follicular Fluid metabolism
Follicular Fluid chemistry
Endometriosis metabolism
Fertilization in Vitro
Metabolome
Subjects
Details
- Language :
- English
- ISSN :
- 1472-6491
- Volume :
- 49
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Reproductive biomedicine online
- Publication Type :
- Academic Journal
- Accession number :
- 38810314
- Full Text :
- https://doi.org/10.1016/j.rbmo.2024.103912