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Protocol for inducing severe Scn2a insufficiency in mice by intracerebroventricular antisense oligonucleotide injection.
- Source :
-
STAR protocols [STAR Protoc] 2024 Jun 21; Vol. 5 (2), pp. 103094. Date of Electronic Publication: 2024 May 25. - Publication Year :
- 2024
-
Abstract
- SCN2A loss-of-function variants cause a range of neurodevelopmental disorders. Here, we present a protocol to induce severe Scn2a insufficiency in mice. We describe steps for intracerebroventricular (ICV) antisense oligonucleotide (ASO) injection that causes a selective downregulation of Scn2a and ASO-mediated mRNA degradation. We then detail procedures for qPCR and western blot protocol to measure Scn2a mRNA and protein. This protocol can be used as a mouse model for behavioral and in vivo two-photon Ca <superscript>2+</superscript> imaging.<br />Competing Interests: Declaration of interests The authors declare no competing interests.<br /> (Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Mice
Disease Models, Animal
Injections, Intraventricular
RNA, Messenger genetics
RNA, Messenger metabolism
NAV1.2 Voltage-Gated Sodium Channel genetics
Oligonucleotides, Antisense administration & dosage
Oligonucleotides, Antisense genetics
Oligonucleotides, Antisense pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 2666-1667
- Volume :
- 5
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- STAR protocols
- Publication Type :
- Academic Journal
- Accession number :
- 38796847
- Full Text :
- https://doi.org/10.1016/j.xpro.2024.103094