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Detection of KPC enzyme by MALDI-TOF MS from bacteria impregnated in filter paper.
- Source :
-
Journal of microbiological methods [J Microbiol Methods] 2024 Aug; Vol. 223, pp. 106962. Date of Electronic Publication: 2024 May 23. - Publication Year :
- 2024
-
Abstract
- The main mechanism that causes resistance to carbapenem, one of the most potent antibiotic available, in Enterobacterales bacterial isolates, is due to Klebsiella pneumoniae carbapenemase (KPC) production by the bacterium. KPC is spread worldwide, requiring laboratories to be capable of identifying this enzyme, however some methods can be expensive for small laboratories, especially in developing countries. Therefore, the development of methods with low cost of reagents for the detection of KPC enzyme is necessary. The objective of this study was to evaluate the detection of KPC enzyme by MALDI-TOF MS from inactivated bacteria impregnated in filter paper. A total of 129 Enterobacterales isolates were impregnated in filter paper, and after 7 days at room temperature, they were subjected to a protein extraction protocol and spectra acquisition, in triplicates, by MALDI-TOF MS. The spectra were evaluated and KPC was identified according to the presence of a peak of 28,712.62 ± 27.80 m/z. Considering the presence of the KPC peak in at least one spectrum of the triplicates, this method presented 60.8% sensitivity and 96.4% specificity. However, considering the presence of KPC peak in at least two spectra of the triplicate, a specificity of 100% was achieved. The detection of KPC enzyme from inactivated bacteria impregnated in filter paper can be used as a method to confirm the presence of KPC, which could be very significant for small laboratories with limited resources.<br />Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.<br /> (Copyright © 2024 Elsevier B.V. All rights reserved.)
- Subjects :
- Paper
Sensitivity and Specificity
Carbapenems pharmacology
Humans
Enterobacteriaceae enzymology
Enterobacteriaceae isolation & purification
Anti-Bacterial Agents pharmacology
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization methods
beta-Lactamases analysis
beta-Lactamases metabolism
Bacterial Proteins analysis
Bacterial Proteins isolation & purification
Klebsiella pneumoniae enzymology
Klebsiella pneumoniae isolation & purification
Subjects
Details
- Language :
- English
- ISSN :
- 1872-8359
- Volume :
- 223
- Database :
- MEDLINE
- Journal :
- Journal of microbiological methods
- Publication Type :
- Academic Journal
- Accession number :
- 38795732
- Full Text :
- https://doi.org/10.1016/j.mimet.2024.106962