Back to Search
Start Over
Serum Amyloid A3 Promoter-Luciferase Reporter Mice Are Useful for Early Drug-Induced Nephrotoxicity Detection.
- Source :
-
International journal of molecular sciences [Int J Mol Sci] 2024 May 08; Vol. 25 (10). Date of Electronic Publication: 2024 May 08. - Publication Year :
- 2024
-
Abstract
- Early detection of drug-induced kidney injury is essential for drug development. In this study, multiple low-dose aristolochic acid (AA) and cisplatin (Cis) injections increased renal mRNA levels of inflammation, fibrosis, and renal tubule injury markers. We applied a serum amyloid A3 (Saa3) promoter-driven luciferase reporter (Saa3 promoter-luc mice) to these two tubulointerstitial nephritis models and performed in vivo bioluminescence imaging to monitor early renal pathologies. The bioluminescent signals from renal tissues with AA or CIS injections were stronger than those from normal kidney tissues obtained from normal mice. To verify whether the visualized bioluminescence signal was specifically generated by the injured kidney, we performed in vivo bioluminescence analysis after opening the stomachs of Saa3 promoter-luc mice, and the Saa3-mediated bioluminescent signal was specifically detected in the injured kidney. This study showed that Saa3 promoter activity is a potent non-invasive indicator for the early detection of drug-induced nephrotoxicity.<br />Competing Interests: The authors declare no conflicts of interest.
- Subjects :
- Animals
Mice
Genes, Reporter
Cisplatin toxicity
Cisplatin adverse effects
Luminescent Measurements methods
Male
Kidney Diseases chemically induced
Kidney Diseases genetics
Kidney Diseases metabolism
Kidney Diseases pathology
Kidney metabolism
Kidney drug effects
Kidney pathology
Disease Models, Animal
Mice, Inbred C57BL
Serum Amyloid A Protein genetics
Serum Amyloid A Protein metabolism
Promoter Regions, Genetic
Luciferases metabolism
Luciferases genetics
Aristolochic Acids toxicity
Subjects
Details
- Language :
- English
- ISSN :
- 1422-0067
- Volume :
- 25
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- International journal of molecular sciences
- Publication Type :
- Academic Journal
- Accession number :
- 38791162
- Full Text :
- https://doi.org/10.3390/ijms25105124