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Comparative analysis, diversification, and functional validation of plant nucleotide-binding site domain genes.
- Source :
-
Scientific reports [Sci Rep] 2024 May 24; Vol. 14 (1), pp. 11930. Date of Electronic Publication: 2024 May 24. - Publication Year :
- 2024
-
Abstract
- Nucleotide-binding site (NBS) domain genes are one of the superfamily of resistance genes involved in plant responses to pathogens. The current study identified 12,820 NBS-domain-containing genes across 34 species covering from mosses to monocots and dicots. These identified genes are classified into 168 classes with several novel domain architecture patterns encompassing significant diversity among plant species. Several classical (NBS, NBS-LRR, TIR-NBS, TIR-NBS-LRR, etc.) and species-specific structural patterns (TIR-NBS-TIR-Cupin&#95;1-Cupin&#95;1, TIR-NBS-Prenyltransf, Sugar&#95;tr-NBS etc.) were discovered. We observed 603 orthogroups (OGs) with some core (most common orthogroups; OG <subscript>0</subscript> , OG <subscript>1</subscript> , OG <subscript>2,</subscript> etc.) and unique (highly specific to species; OG <subscript>80</subscript> , OG <subscript>82,</subscript> etc.) OGs with tandem duplications. The expression profiling presented the putative upregulation of OG <subscript>2</subscript> , OG <subscript>6,</subscript> and OG <subscript>15</subscript> in different tissues under various biotic and abiotic stresses in susceptible and tolerant plants to cotton leaf curl disease (CLCuD). The genetic variation between susceptible (Coker 312) and tolerant (Mac7) Gossypium hirsutum accessions identified several unique variants in NBS genes of Mac7 (6583 variants) and Coker312 (5173 variants). The protein-ligand and proteins-protein interaction showed a strong interaction of some putative NBS proteins with ADP/ATP and different core proteins of the cotton leaf curl disease virus. The silencing of GaNBS (OG <subscript>2</subscript> ) in resistant cotton through virus-induced gene silencing (VIGS) demonstrated its putative role in virus tittering. The presented study will be further helpful in understanding the plant adaptation mechanism.<br /> (© 2024. The Author(s).)
- Subjects :
- Binding Sites
Nucleotides genetics
Nucleotides metabolism
Disease Resistance genetics
Gene Expression Regulation, Plant
Plant Diseases genetics
Plant Diseases virology
Genes, Plant
Phylogeny
Plants genetics
Gene Expression Profiling
Protein Domains
Plant Proteins genetics
Plant Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 14
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 38789717
- Full Text :
- https://doi.org/10.1038/s41598-024-62876-5