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Molecular characterization and function of hif1a and fih1 in response to acute thermal stress in American shad (Alosa sapidissima).
- Source :
-
Fish physiology and biochemistry [Fish Physiol Biochem] 2024 Aug; Vol. 50 (4), pp. 1563-1581. Date of Electronic Publication: 2024 May 24. - Publication Year :
- 2024
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Abstract
- In order to evaluate the function of hypoxia-inducible factor-1 alpha (hif1α) and factor inhibiting hif1α (fih1) in response to thermal stress, we first conducted a functional analysis of A. sapidissima hif1α and fih1, and determined hif1α and fih1 expressions in different tissues in response to thermal stress based on identified housekeeping genes (HKGs). The results showed that hif1α and fih1 were mainly located in the nucleus and cytoplasm. The full length cDNA sequence of hif1α and fih1 was 4073 bp and 2759 bp, respectively. The cDNA sequence of hif1α includes 15 exons encoding 750 amino acid residues, and the full length cDNA sequence of fih1 contains 9 exons encoding 354 amino acid residues. During the acute thermal stress transferring from 16 ± 0.5 °C (control) to 20 ± 0.5 °C, 25 ± 0.5 °C, and 30 ± 0.5 °C for 15 min, it was found that the expression trends of hif1α and fih1 showed an inhibitory regulation in the heart, while they consistently expressed in brain, intestine, muscle, gill, kidney and liver. In conclusion, this is the first study to identify the tissue-specific HKGs in A. sapidissima and found that ef1α and β-actin are the most suitable HKGs. Hif1α and Fih1 are mainly the nuclear and cytoplasmic proteins, respectively, having high levels in the heart and brain. Alosa sapidissima countered a temperature increase from 16 to 25 ℃ by regulating the expressions of hif1α and fih1, but their physiological regulatory functions were unable to cope with acute thermal stress when the temperature difference was 14 ℃ (from 16 to 30 ℃).<br /> (© 2024. The Author(s), under exclusive licence to Springer Nature B.V.)
- Subjects :
- Animals
Fish Proteins genetics
Fish Proteins metabolism
Stress, Physiological physiology
Amino Acid Sequence
Gene Expression Regulation
Heat-Shock Response physiology
Heat-Shock Response genetics
Hot Temperature
Phylogeny
DNA, Complementary genetics
Base Sequence
Hypoxia-Inducible Factor 1, alpha Subunit genetics
Hypoxia-Inducible Factor 1, alpha Subunit metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1573-5168
- Volume :
- 50
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Fish physiology and biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 38789648
- Full Text :
- https://doi.org/10.1007/s10695-024-01356-z