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Ectopic MYBL2-Mediated Regulation of Androglobin Gene Expression.
- Source :
-
Cells [Cells] 2024 May 11; Vol. 13 (10). Date of Electronic Publication: 2024 May 11. - Publication Year :
- 2024
-
Abstract
- Androglobin (ADGB) is a highly conserved and recently identified member of the globin superfamily. Although previous studies revealed a link to ciliogenesis and an involvement in murine spermatogenesis, its physiological function remains mostly unknown. Apart from FOXJ1-dependent regulation, the transcriptional landscape of the ADGB gene remains unexplored. We, therefore, aimed to obtain further insights into regulatory mechanisms governing ADGB expression. To this end, changes in ADGB promoter activity were examined using luciferase reporter gene assays in the presence of a set of more than 475 different exogenous transcription factors. MYBL2 and PITX2 resulted in the most pronounced increase in ADGB promoter-dependent luciferase activity. Subsequent truncation strategies of the ADGB promoter fragment narrowed down the potential MYBL2 and PITX2 binding sites within the proximal ADGB promoter. Furthermore, MYBL2 binding sites on the ADGB promoter were further validated via a guide RNA-mediated interference strategy using reporter assays. Chromatin immunoprecipitation (ChIP)-qPCR experiments illustrated enrichment of the endogenous ADGB promoter region upon MYBL2 and PITX2 overexpression. Consistently, ectopic MYBL2 expression induced endogenous ADGB mRNA levels. Collectively, our data indicate that ADGB is strongly regulated at the transcriptional level and might have functions beyond ciliogenesis.
- Subjects :
- Humans
Binding Sites
Ectopic Gene Expression
Globins genetics
Globins metabolism
Homeobox Protein PITX2
Homeodomain Proteins genetics
Homeodomain Proteins metabolism
Protein Binding
Cell Cycle Proteins genetics
Cell Cycle Proteins metabolism
Gene Expression Regulation
Promoter Regions, Genetic genetics
Transcription Factors metabolism
Transcription Factors genetics
Trans-Activators genetics
Trans-Activators metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 2073-4409
- Volume :
- 13
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Cells
- Publication Type :
- Academic Journal
- Accession number :
- 38786048
- Full Text :
- https://doi.org/10.3390/cells13100826