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Development of a DNA-Based Lateral Flow Strip Membrane Assay for Rapid Screening and Genotyping of Six High-Incidence STD Pathogens.
- Source :
-
Biosensors [Biosensors (Basel)] 2024 May 20; Vol. 14 (5). Date of Electronic Publication: 2024 May 20. - Publication Year :
- 2024
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Abstract
- Sexually transmitted diseases (STDs) are a global concern because approximately 1 million new cases emerge daily. Most STDs are curable, but if left untreated, they can cause severe long-term health implications, including infertility and even death. Therefore, a test enabling rapid and accurate screening and genotyping of STD pathogens is highly awaited. Herein, we present the development of the DNA-based 6STD Genotyping 9G Membrane test, a lateral flow strip membrane assay, for the detection and genotyping of six STD pathogens, including Trichomonas vaginalis , Ureaplasma urealyticum , Neisseria gonorrhoeae , Chlamydia trachomatis , Mycoplasma hominis , and Mycoplasma genitalium . Here, we developed a multiplex PCR primer set that allows PCR amplification of genomic materials for these six STD pathogens. We also developed the six ssDNA probes that allow highly efficient detection of the six STD pathogens. The 6STD Genotyping 9G Membrane test lets us obtain the final detection and genotyping results in less than 30 m after PCR at 25 °C. The accuracy of the 6STD Genotyping 9G membrane test in STD genotyping was confirmed by its 100% concordance with the sequencing results of 120 clinical samples. Therefore, the 6STD Genotyping 9G Membrane test emerges as a promising diagnostic tool for precise STD genotyping, facilitating informed decision-making in clinical practice.
- Subjects :
- Humans
Trichomonas vaginalis genetics
Trichomonas vaginalis isolation & purification
Genotyping Techniques
Mycoplasma hominis isolation & purification
Mycoplasma hominis genetics
Ureaplasma urealyticum genetics
Ureaplasma urealyticum isolation & purification
DNA
Mycoplasma genitalium genetics
Mycoplasma genitalium isolation & purification
Biosensing Techniques
DNA, Bacterial analysis
Multiplex Polymerase Chain Reaction methods
Chlamydia trachomatis genetics
Chlamydia trachomatis isolation & purification
Neisseria gonorrhoeae genetics
Neisseria gonorrhoeae isolation & purification
Sexually Transmitted Diseases microbiology
Sexually Transmitted Diseases diagnosis
Genotype
Subjects
Details
- Language :
- English
- ISSN :
- 2079-6374
- Volume :
- 14
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Biosensors
- Publication Type :
- Academic Journal
- Accession number :
- 38785734
- Full Text :
- https://doi.org/10.3390/bios14050260