Foliar spraying of zinc oxide nanoparticles improves water transport and nitrogen metabolism in tomato (Solanum lycopersicum L.) seedlings mitigating the negative impacts of cadmium.

The use of bio-nanotechnology in agriculture-such as the biological applications of metal oxide nanoparticles (NPs)-greatly improves crop yield and quality under different abiotic stress factors including soil metal contamination. Here, we explore the effectiveness of zinc oxide (ZnO)-NPs (0, 50 mg/L) foliar spraying to ameliorate the detrimental effects of cadmium (Cd) on the water transport and nitrogen metabolism in tomato (Solanum lycopersicum Mill. cv. Chibli F1) plants grown on a Cd-supplied (CdCl ₂ ; 0, 10, 40 μM) Hoagland nutrient solution. The results depicted that the individually studied factors (ZnO-NPs and Cd) had a significant impact on all the physiological parameters analyzed. Independently to the Cd concentration, ZnO-NPs-sprayed plants showed significantly higher dry weight (DW) in both leaves and roots compared to the non-sprayed ones, which was in consonance with higher and lower levels of Zn ²⁺ and Cd ²⁺ ions, respectively, in these organs. Interestingly, ZnO-NPs spraying improved water status in all Cd-treated plants as evidenced by the increase in root hydraulic conductance (L ₀ ), apoplastic water pathway percentage, and leaf and root relative water content (RWC), compared to the non-sprayed plants. This improved water balance was associated with a significant accumulation of osmoprotectant osmolytes, such as proline and soluble sugars in the plant organs, reducing electrolyte leakage (EL), and osmotic potential (ψ _π ). Also, ZnO-NPs spraying significantly improved NO ₃ ^- and NH ₄ ⁺ assimilation in the leaf and root tissues of all Cd-treated plants, leading to a reduction in NH ₄ ⁺ toxicity. Our findings point out new insights into how ZnO-NPs affect water transport and nitrogen metabolism in Cd-stressed plants and support their use to improve crop resilience against Cd-contaminated soils.
(© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)