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A positive affect intervention alters leukocyte DNA methylation in sexual minority men with HIV who use methamphetamine.
- Source :
-
Brain, behavior, and immunity [Brain Behav Immun] 2024 Aug; Vol. 120, pp. 151-158. Date of Electronic Publication: 2024 May 20. - Publication Year :
- 2024
-
Abstract
- Objective: This epigenomics sub-study embedded within a randomized controlled trial examined whether an evidenced-based behavioral intervention model that decreased stimulant use altered leukocyte DNA methylation (DNAm).<br />Methods: Sexual minority men with HIV who use methamphetamine were randomized to a five-session positive affect intervention (n = 32) or an attention-control condition (n = 21), both delivered during three months of contingency management for stimulant abstinence. All participants exhibited sustained HIV virologic control - an HIV viral load less than 40 copies/mL at baseline and six months post-randomization. The Illumina EPIC BeadChip measured leukocyte methylation of cytosine-phosphate-guanosine (CpG) sites mapping onto five a priori candidate genes of interest (i.e., ADRB2, BDNF, FKBP5, NR3C1, OXTR). Functional DNAm pathways and soluble markers of immune dysfunction were secondary outcomes.<br />Results: Compared to the attention-control condition, the positive affect intervention significantly decreased methylation of CpG sites on genes that regulate β <subscript>2</subscript> adrenergic and oxytocin receptors. There was an inconsistent pattern for the direction of the intervention effects on methylation of CpG sites on genes for glucocorticoid receptors and brain-derived neurotrophic factor. Pathway analyses adjusting for the false discovery rate (p <subscript>adj</subscript>  < 0.05) revealed significant intervention-related alterations in DNAm of Reactome pathways corresponding to neural function as well as dopamine, glutamate, and serotonin release. Positive affect intervention effects on DNAm were accompanied by significant reductions in the self-reported frequency of stimulant use.<br />Conclusions: There is an epigenetic signature of an evidence-based behavioral intervention model that reduced stimulant use, which will guide the identification of biomarkers for treatment responses.<br />Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.<br /> (Copyright © 2024 Elsevier Inc. All rights reserved.)
- Subjects :
- Humans
Male
Adult
Middle Aged
Epigenesis, Genetic
Brain-Derived Neurotrophic Factor genetics
Brain-Derived Neurotrophic Factor metabolism
Tacrolimus Binding Proteins genetics
Affect drug effects
Amphetamine-Related Disorders genetics
Receptors, Glucocorticoid genetics
Receptors, Glucocorticoid metabolism
Behavior Therapy methods
Receptors, Oxytocin genetics
DNA Methylation
Methamphetamine
HIV Infections genetics
HIV Infections drug therapy
Leukocytes metabolism
Leukocytes drug effects
Sexual and Gender Minorities
Subjects
Details
- Language :
- English
- ISSN :
- 1090-2139
- Volume :
- 120
- Database :
- MEDLINE
- Journal :
- Brain, behavior, and immunity
- Publication Type :
- Academic Journal
- Accession number :
- 38777283
- Full Text :
- https://doi.org/10.1016/j.bbi.2024.05.025