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Immunization with a Mu-class glutathione transferase from Echinococcus granulosus induces efficient antibody responses and confers long-term protection against secondary cystic echinococcosis.
- Source :
-
Microbes and infection [Microbes Infect] 2024 Jul-Aug; Vol. 26 (5-6), pp. 105364. Date of Electronic Publication: 2024 May 21. - Publication Year :
- 2024
-
Abstract
- Cystic echinococcosis, a zoonosis caused by cestodes belonging to the Echinococcus granulosus sensu lato (s.l.) genetic complex, affects humans and diverse livestock species. Although a veterinary vaccine exhibiting high levels of antibody-mediated protection has successfully reached the market, the large genetic diversity among parasite isolates and their particular host preferences, makes still necessary the search for novel vaccine candidates. Glutathione transferases (GSTs) constitute attractive targets for immunoprophylaxis due to their outstanding relevance in helminth detoxification processes, against both exogenous and endogenous stressors. Among the six GSTs known to be expressed in E. granulosus s.l., EgGST1 (Mu-class), EgGST2 (Sigma-class), and EgGST3 (a still non-classifiable isoenzyme), show the highest proteomic expression. Therefore, their recombinant forms -rEgGST1, rEgGST2 and rEgGST3- were herein analyzed regarding their potential to induce long-term antiparasite protection in mice. Only immunization with rEgGST1 induced long-lasting protection; and accordingly, rEgGST1-specific antibodies enhanced the parasite killing through both the classical activation of the host complement system and the antibody-dependent cellular cytotoxicity by macrophages. These results support further testing of rEgGST1 as a vaccine candidate in diverse hosts due to the broad expression of EgGST1 in different parasite stages and tissues.<br />Competing Interests: Declaration of competing interest The authors declare that they have no conflict of interests.<br /> (Copyright © 2024 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.)
- Subjects :
- Animals
Mice
Antibody Formation immunology
Female
Mice, Inbred BALB C
Immunization
Helminth Proteins immunology
Helminth Proteins genetics
Echinococcus granulosus immunology
Echinococcus granulosus genetics
Echinococcus granulosus enzymology
Echinococcosis prevention & control
Echinococcosis immunology
Echinococcosis parasitology
Glutathione Transferase immunology
Glutathione Transferase genetics
Glutathione Transferase metabolism
Antibodies, Helminth immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1769-714X
- Volume :
- 26
- Issue :
- 5-6
- Database :
- MEDLINE
- Journal :
- Microbes and infection
- Publication Type :
- Academic Journal
- Accession number :
- 38777107
- Full Text :
- https://doi.org/10.1016/j.micinf.2024.105364