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Bridging fungal resistance and plant growth through constitutive overexpression of Thchit42 gene in Pelargonium graveolens.
- Source :
-
Plant cell reports [Plant Cell Rep] 2024 May 21; Vol. 43 (6), pp. 147. Date of Electronic Publication: 2024 May 21. - Publication Year :
- 2024
-
Abstract
- Key Message: Thchit42 constitutive expression for fungal resistance showed synchronisation with leaf augmentation and transcriptome analysis revealed the Longifolia and Zinc finger RICESLEEPER gene is responsible for plant growth and development. Pelargonium graveolens essential oil possesses significant attributes, known for perfumery and aromatherapy. However, optimal yield and propagation are predominantly hindered by biotic stress. All biotechnological approaches have yet to prove effective in addressing fungal resistance. The current study developed transgenic geranium bridging molecular mechanism of fungal resistance and plant growth by introducing cassette 35S::Thchit42. Furthermore, 120 independently putative transformed explants were regenerated on kanamycin fortified medium. Primarily transgenic lines were demonstrated peak pathogenicity and antifungal activity against formidable Colletotrichum gloeosporioides and Fusarium oxysporum. Additionally, phenotypic analysis revealed ~ 2fold increase in leaf size and ~ 2.1fold enhanced oil content. To elucidate the molecular mechanisms for genotypic cause, de novo transcriptional profiles were analyzed to indicate that the auxin-regulated longifolia gene is accountable for augmentation in leaf size, and zinc finger (ZF) RICESLEEPER attributes growth upregulation. Collectively, data provides valuable insights into unravelling the mechanism of Thchit42-mediated crosstalk between morphological and chemical alteration in transgenic plants. This knowledge might create novel opportunities to cultivate fungal-resistant geranium throughout all seasons to fulfil demand.<br /> (© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
- Subjects :
- Plant Proteins genetics
Plant Proteins metabolism
Plant Diseases microbiology
Plant Diseases genetics
Colletotrichum pathogenicity
Colletotrichum physiology
Oils, Volatile metabolism
Oils, Volatile pharmacology
Geranium genetics
Pelargonium genetics
Plants, Genetically Modified
Fusarium pathogenicity
Fusarium physiology
Disease Resistance genetics
Gene Expression Regulation, Plant
Plant Leaves genetics
Plant Leaves microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 1432-203X
- Volume :
- 43
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Plant cell reports
- Publication Type :
- Academic Journal
- Accession number :
- 38771491
- Full Text :
- https://doi.org/10.1007/s00299-024-03233-8