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Huoxue Jiedu Huayu recipe inhibits macrophage-secreted vascular endothelial growth factor-a on angiogenesis and alleviates renal fibrosis in the contralateral kidneys of unilateral ureteral obstruction rats.
- Source :
-
Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan [J Tradit Chin Med] 2024 Jun; Vol. 44 (3), pp. 458-467. - Publication Year :
- 2024
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Abstract
- OBJECTIVE:To elucidate the mechanism by which Huoxue Jiedu Huayu recipe (, HJHR) regulates angiogenesis in the contralateral kidney of unilateral ureteral obstruction (UUO) rats and the mechanism by which it reduces of renal fibrosis.<br />Methods: Male Wistar rats were randomly divided into 4 groups: the sham group, UUO group (180 d of left ureter ligation), UUO plus eplerenone (EPL) group, and UUO plus HJHR group. After 180 d of oral drug administration, blood and contralateral kidneys were collected for analysis. Angiogenesis- and fibrosis-related indexes were detected.<br />Results: HJHR and EPL improved structural damage and renal interstitial fibrosis in the contralateral kidney and reduced the protein expression levels of α-smooth muscle actin (α-SMA), vimentin and collagen I. Moreover, these treatments could reduce the expression of vascular endothelial growth factor-A (VEGFA) by inhibiting the infiltration of macrophages. Furthermore, HJHR and EPL significantly reduced the expression of CD34 and CD105 by downregulating VEGFA production, which inhibited angiogenesis. Finally, the coexpressions of CD34, CD105 and α-SMA were decreased in the HJHR and EPL groups, indicating that endothelial-to-mesenchymal transition was inhibited.<br />Conclusions: These findings confirm that HJHR alleviates contralateral renal fibrosis by inhibiting VEGFA-induced angiogenesis, encourage the use of HJHR against renal interstitial fibrosis and provide a theoretical basis for the clinical management of patients with CKD.
- Subjects :
- Animals
Male
Rats
Humans
Neovascularization, Pathologic drug therapy
Neovascularization, Pathologic metabolism
Kidney Diseases drug therapy
Kidney Diseases metabolism
Kidney Diseases etiology
Kidney Diseases genetics
Angiogenesis
Ureteral Obstruction metabolism
Ureteral Obstruction drug therapy
Ureteral Obstruction genetics
Rats, Wistar
Vascular Endothelial Growth Factor A metabolism
Vascular Endothelial Growth Factor A genetics
Fibrosis
Kidney drug effects
Kidney metabolism
Macrophages drug effects
Macrophages metabolism
Drugs, Chinese Herbal administration & dosage
Subjects
Details
- Language :
- English
- ISSN :
- 2589-451X
- Volume :
- 44
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan
- Publication Type :
- Academic Journal
- Accession number :
- 38767629
- Full Text :
- https://doi.org/10.19852/j.cnki.jtcm.20240423.005