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Accelerated diagnosis: a crosslinking catalytic hairpin assembly system for rapid and sensitive SARS-CoV-2 RNA detection.
- Source :
-
Mikrochimica acta [Mikrochim Acta] 2024 May 16; Vol. 191 (6), pp. 333. Date of Electronic Publication: 2024 May 16. - Publication Year :
- 2024
-
Abstract
- The COVID-19 pandemic has underscored the urgent need for rapid and reliable strategies for early detection of SARS-CoV-2. In this study, we propose a DNA nanosphere-based crosslinking catalytic hairpin assembly (CCHA) system for the rapid and sensitive SARS-CoV-2 RNA detection. The CCHA system employs two DNA nanospheres functionalized with catalytic hairpin assembly (CHA) hairpins. The presence of target SARS-CoV-2 RNA initiated the crosslinking of DNA nanospheres via CHA process, leading to the amplification of fluorescence signals. As a result, the speed of SARS-CoV-2 diagnosis was enhanced by significantly increasing the local concentration of the reagents in a crosslinked DNA product, leading to a detection limit of 363 fM within 5 min. The robustness of this system has been validated in complex environments, such as fetal bovine serum and saliva. Hence, the proposed CCHA system offers an efficient and simple approach for rapid detection of SARS-CoV-2 RNA, holding substantial promise for enhancing COVID-19 diagnosis.<br /> (© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.)
- Subjects :
- Humans
Nanospheres chemistry
DNA chemistry
Inverted Repeat Sequences
Animals
COVID-19 Nucleic Acid Testing methods
Cattle
Cross-Linking Reagents chemistry
Saliva virology
SARS-CoV-2 genetics
SARS-CoV-2 isolation & purification
RNA, Viral analysis
RNA, Viral genetics
COVID-19 diagnosis
COVID-19 virology
Limit of Detection
Subjects
Details
- Language :
- English
- ISSN :
- 1436-5073
- Volume :
- 191
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Mikrochimica acta
- Publication Type :
- Academic Journal
- Accession number :
- 38753167
- Full Text :
- https://doi.org/10.1007/s00604-024-06396-z