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Development of novel iron(III) crosslinked bioinks comprising carboxymethyl cellulose, xanthan gum, and hyaluronic acid for soft tissue engineering applications.
- Source :
-
Journal of materials chemistry. B [J Mater Chem B] 2024 Jul 10; Vol. 12 (27), pp. 6627-6642. Date of Electronic Publication: 2024 Jul 10. - Publication Year :
- 2024
-
Abstract
- The advent of three-dimensional (3D) bioprinting offers a feasible approach to construct complex structures for soft tissue regeneration. Carboxymethyl cellulose (CMC) has been emerging as a very promising biomaterial for 3D bioprinting. However, due to the inability to maintain the post-printed stability, CMC needs to be physically blended and/or chemically crosslinked with other polymers. In this context, this study presents the combination of CMC with xanthan gum (XG) and hyaluronic acid (HA) to formulate a multicomponent bioink, leveraging the printability of CMC and XG, as well as the cellular support properties of HA. The ionic crosslinking of printed constructs with iron(III) via the metal-ion coordination between ferric cations and carboxylate groups of the three polymers was introduced to induce improved mechanical strength and long-term stability. Moreover, immortalized human epidermal keratinocytes (HaCaT) and human foreskin fibroblasts (HFF) encapsulated within iron-crosslinked printed hydrogels exhibited excellent cell viability (more than 95%) and preserved morphology. Overall, the presented study highlights that the combination of these three biopolymers and the ionic crosslinking with ferric ions is a valuable strategy to be considered for the development of new and advanced hydrogel-based bioinks for soft tissue engineering applications.
- Subjects :
- Humans
Cross-Linking Reagents chemistry
Biocompatible Materials chemistry
Biocompatible Materials pharmacology
Biocompatible Materials chemical synthesis
Cell Survival drug effects
Hydrogels chemistry
Hydrogels pharmacology
Hydrogels chemical synthesis
Iron chemistry
Ink
Fibroblasts drug effects
Fibroblasts cytology
Bioprinting
Keratinocytes drug effects
Keratinocytes cytology
Hyaluronic Acid chemistry
Hyaluronic Acid pharmacology
Polysaccharides, Bacterial chemistry
Carboxymethylcellulose Sodium chemistry
Tissue Engineering
Subjects
Details
- Language :
- English
- ISSN :
- 2050-7518
- Volume :
- 12
- Issue :
- 27
- Database :
- MEDLINE
- Journal :
- Journal of materials chemistry. B
- Publication Type :
- Academic Journal
- Accession number :
- 38752707
- Full Text :
- https://doi.org/10.1039/d4tb00142g