Back to Search Start Over

Development of novel iron(III) crosslinked bioinks comprising carboxymethyl cellulose, xanthan gum, and hyaluronic acid for soft tissue engineering applications.

Authors :
Le HP
Hassan K
Ramezanpour M
Campbell JA
Tung TT
Vreugde S
Losic D
Source :
Journal of materials chemistry. B [J Mater Chem B] 2024 Jul 10; Vol. 12 (27), pp. 6627-6642. Date of Electronic Publication: 2024 Jul 10.
Publication Year :
2024

Abstract

The advent of three-dimensional (3D) bioprinting offers a feasible approach to construct complex structures for soft tissue regeneration. Carboxymethyl cellulose (CMC) has been emerging as a very promising biomaterial for 3D bioprinting. However, due to the inability to maintain the post-printed stability, CMC needs to be physically blended and/or chemically crosslinked with other polymers. In this context, this study presents the combination of CMC with xanthan gum (XG) and hyaluronic acid (HA) to formulate a multicomponent bioink, leveraging the printability of CMC and XG, as well as the cellular support properties of HA. The ionic crosslinking of printed constructs with iron(III) via the metal-ion coordination between ferric cations and carboxylate groups of the three polymers was introduced to induce improved mechanical strength and long-term stability. Moreover, immortalized human epidermal keratinocytes (HaCaT) and human foreskin fibroblasts (HFF) encapsulated within iron-crosslinked printed hydrogels exhibited excellent cell viability (more than 95%) and preserved morphology. Overall, the presented study highlights that the combination of these three biopolymers and the ionic crosslinking with ferric ions is a valuable strategy to be considered for the development of new and advanced hydrogel-based bioinks for soft tissue engineering applications.

Details

Language :
English
ISSN :
2050-7518
Volume :
12
Issue :
27
Database :
MEDLINE
Journal :
Journal of materials chemistry. B
Publication Type :
Academic Journal
Accession number :
38752707
Full Text :
https://doi.org/10.1039/d4tb00142g