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Bioinformatics approach combined with experimental verification reveals OAS3 gene implicated in paclitaxel resistance in head and neck cancer.
- Source :
-
Head & neck [Head Neck] 2024 Sep; Vol. 46 (9), pp. 2178-2196. Date of Electronic Publication: 2024 May 16. - Publication Year :
- 2024
-
Abstract
- Background: This study aimed to identify a candidate gene associated with paclitaxel (PTX) resistance and to evaluate functionally its biological role in the PTX-resistant head and neck squamous cell carcinoma (HNSCC) cell lines and clinical specimens.<br />Methods: Microarray data series containing samples of different types of cancers resistant to PTX were analyzed and then a candidate gene associated with PTX resistance was identified using various bioinformatics tools. After the suppression of the target gene expression, changes in cell viability and colony-forming ability were evaluated in PTX-resistant FaDu and SCC-9 cell lines.<br />Results: Bioinformatics analyses of upregulated genes in PTX-resistant cancer cells indicated that OAS3 was associated with PTX resistance. The downregulation of OAS3 expression significantly reduced the viability and colony-forming capacity of PTX-resistant SCC-9 cells by inducing apoptosis and cell cycle arrest at G0/G1 phase.<br />Conclusions: The therapeutic targeting of OAS3 may resensitize PTX-resistant HNSCC cells with high OAS3 expression to PTX treatment.<br /> (© 2024 The Authors. Head & Neck published by Wiley Periodicals LLC.)
- Subjects :
- Humans
Cell Line, Tumor
Squamous Cell Carcinoma of Head and Neck genetics
Squamous Cell Carcinoma of Head and Neck drug therapy
Squamous Cell Carcinoma of Head and Neck pathology
Antineoplastic Agents, Phytogenic pharmacology
Antineoplastic Agents, Phytogenic therapeutic use
Gene Expression Regulation, Neoplastic
Apoptosis drug effects
Apoptosis genetics
2',5'-Oligoadenylate Synthetase genetics
Male
Female
Cell Survival drug effects
Cell Survival genetics
Middle Aged
Paclitaxel pharmacology
Paclitaxel therapeutic use
Drug Resistance, Neoplasm genetics
Head and Neck Neoplasms genetics
Head and Neck Neoplasms drug therapy
Head and Neck Neoplasms pathology
Computational Biology
Carcinoma, Squamous Cell genetics
Carcinoma, Squamous Cell drug therapy
Carcinoma, Squamous Cell pathology
Subjects
Details
- Language :
- English
- ISSN :
- 1097-0347
- Volume :
- 46
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Head & neck
- Publication Type :
- Academic Journal
- Accession number :
- 38752376
- Full Text :
- https://doi.org/10.1002/hed.27803