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Bioinformatics approach combined with experimental verification reveals OAS3 gene implicated in paclitaxel resistance in head and neck cancer.

Authors :
Caglar HO
Aytatli A
Barlak N
Aydin Karatas E
Tatar A
Sahin A
Karatas OF
Source :
Head & neck [Head Neck] 2024 Sep; Vol. 46 (9), pp. 2178-2196. Date of Electronic Publication: 2024 May 16.
Publication Year :
2024

Abstract

Background: This study aimed to identify a candidate gene associated with paclitaxel (PTX) resistance and to evaluate functionally its biological role in the PTX-resistant head and neck squamous cell carcinoma (HNSCC) cell lines and clinical specimens.<br />Methods: Microarray data series containing samples of different types of cancers resistant to PTX were analyzed and then a candidate gene associated with PTX resistance was identified using various bioinformatics tools. After the suppression of the target gene expression, changes in cell viability and colony-forming ability were evaluated in PTX-resistant FaDu and SCC-9 cell lines.<br />Results: Bioinformatics analyses of upregulated genes in PTX-resistant cancer cells indicated that OAS3 was associated with PTX resistance. The downregulation of OAS3 expression significantly reduced the viability and colony-forming capacity of PTX-resistant SCC-9 cells by inducing apoptosis and cell cycle arrest at G0/G1 phase.<br />Conclusions: The therapeutic targeting of OAS3 may resensitize PTX-resistant HNSCC cells with high OAS3 expression to PTX treatment.<br /> (© 2024 The Authors. Head & Neck published by Wiley Periodicals LLC.)

Details

Language :
English
ISSN :
1097-0347
Volume :
46
Issue :
9
Database :
MEDLINE
Journal :
Head & neck
Publication Type :
Academic Journal
Accession number :
38752376
Full Text :
https://doi.org/10.1002/hed.27803