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Disulfide-adducts with cysteine residues in human serum albumin prove exposure to malodorous mercaptans in vitro.
- Source :
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Analytical biochemistry [Anal Biochem] 2024 Sep; Vol. 692, pp. 115568. Date of Electronic Publication: 2024 May 16. - Publication Year :
- 2024
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Abstract
- Malodorants are mixtures containing mercaptans, which trigger the flight instinct upon exposure and might thus be deployed in military and civilian defense scenarios. Exposure to mercaptans might lead to unconsciousness, thus representing a possible threat for health. Therefore, we developed and validated a bioanalytical procedure for the simultaneous detection and identification of corresponding biomarkers for the verification of exposure to mercaptans. Disulfide-adducts of ethyl mercaptan (SEt), n-butyl mercaptan (S <superscript>n</superscript> Bu), tert-butyl mercaptan (S <superscript>t</superscript> Bu) and iso-amyl mercaptan (S <superscript>i</superscript> Am) with cysteine (Cys) residues in human serum albumin (HSA) were formed by in vitro incubation of human plasma. After pronase-catalyzed proteolysis, reaction products were identified as adducts of the single amino acid Cys and the dipeptide cysteine-proline (Cys <superscript>34</superscript> Pro) detected by a sensitive μLC-ESI MS/MS method working in the scheduled multiple reaction monitoring (sMRM) mode. Dose-response studies showed linearity for the yield of Cys <superscript>34</superscript> Pro-adducts in the range from 6 nM to 300 μM of mercaptans in plasma and limits of identification (LOI) were in the range from 60 nM to 6 μM. Cys <superscript>34</superscript> -adducts showed stability for at least 6 days in plasma (37 °C). The presented disulfide-biomarkers expand the spectrum for bioanalytical verification procedures and might be helpful to prove exposure to malodorants.<br />Competing Interests: Declaration of competing interest None.<br /> (Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.)
Details
- Language :
- English
- ISSN :
- 1096-0309
- Volume :
- 692
- Database :
- MEDLINE
- Journal :
- Analytical biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 38750681
- Full Text :
- https://doi.org/10.1016/j.ab.2024.115568