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Novel approach to soft tissue regeneration: in vitro study of compound hyaluronic acid and horizontal platelet-rich fibrin combination.
- Source :
-
Journal of applied oral science : revista FOB [J Appl Oral Sci] 2024 May 10; Vol. 32, pp. e20230294. Date of Electronic Publication: 2024 May 10 (Print Publication: 2024). - Publication Year :
- 2024
-
Abstract
- Objective: This study aims to develop a compound biomaterial to achieve effective soft tissue regeneration.<br />Methodology: Compound hyaluronic acid (CHA) and liquid horizontal-platelet-rich fibrin (H-PRF) were mixed at a ratio of 1:1 to form a CHA-PRF gel. Human gingival fibroblasts (HGFs) were used in this study. The effect of CHA, H-PRF, and the CHA-PRF gel on cell viability was evaluated by CCK-8 assays. Then, the effect of CHA, H-PRF, and the CHA-PRF gel on collagen formation and deposition was evaluated by qRT‒PCR and immunofluorescence analysis. Finally, qRT‒PCR, immunofluorescence analysis, Transwell assays, and scratch wound-healing assays were performed to determine how CHA, H-PRF, and the CHA-PRF gel affect the migration of HGFs.<br />Results: The combination of CHA and H-PRF shortened the coagulation time of liquid H-PRF. Compared to the pure CHA and H-PRF group, the CHA-PRF group exhibited the highest cell proliferation at all time points, as shown by the CCK-8 assay. Col1a and FAK were expressed at the highest levels in the CHA-PRF group, as shown by qRT‒PCR. CHA and PRF could stimulate collagen formation and HGF migration, as observed by fluorescence microscopy analysis of COL1 and F-actin and Transwell and scratch healing assays.<br />Conclusion: The CHA-PRF group exhibited greater potential to promote soft tissue regeneration by inducing cell proliferation, collagen synthesis, and migration in HGFs than the pure CHA or H-PRF group. CHA-PRF can serve as a great candidate for use alone or in combination with autografts in periodontal or peri-implant soft tissue regeneration.
- Subjects :
- Humans
Cells, Cultured
Time Factors
Reproducibility of Results
Fluorescent Antibody Technique
Real-Time Polymerase Chain Reaction
Collagen
Materials Testing
Wound Healing drug effects
Biocompatible Materials pharmacology
Collagen Type I analysis
Hyaluronic Acid pharmacology
Platelet-Rich Fibrin
Fibroblasts drug effects
Gingiva drug effects
Gingiva cytology
Cell Proliferation drug effects
Cell Survival drug effects
Regeneration drug effects
Cell Movement drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1678-7765
- Volume :
- 32
- Database :
- MEDLINE
- Journal :
- Journal of applied oral science : revista FOB
- Publication Type :
- Academic Journal
- Accession number :
- 38747782
- Full Text :
- https://doi.org/10.1590/1678-7757-2023-0294