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Delayed gramicidin delivery through an intra-pipette capillary facilitates perforated patch recordings.
- Source :
-
Neuroscience research [Neurosci Res] 2024 Oct; Vol. 207, pp. 45-48. Date of Electronic Publication: 2024 May 11. - Publication Year :
- 2024
-
Abstract
- The gramicidin-perforated patch-clamp technique is indispensable for recording neuronal activities without changing the intracellular Cl <superscript>-</superscript> concentration. Conventionally, gramicidin contained in the pipette fluid is delivered to the cell membrane by passive diffusion. Gramicidin deposited on the pipette orifice sometimes hampers giga-seal formation, and perforation progresses only slowly. These problems may be circumvented by delivering a high concentration of gramicidin from an intra-pipette capillary after a giga-seal is formed. We herein describe the detailed protocol of this improved method. This protocol would greatly facilitate the investigation of Cl <superscript>-</superscript> gradient-dependent neuronal activities.<br /> (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)
Details
- Language :
- English
- ISSN :
- 1872-8111
- Volume :
- 207
- Database :
- MEDLINE
- Journal :
- Neuroscience research
- Publication Type :
- Academic Journal
- Accession number :
- 38740268
- Full Text :
- https://doi.org/10.1016/j.neures.2024.05.002