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Melatonin application during cryopreservation improves the development and clinical outcomes of human vitrified-warmed oocytes.
- Source :
-
Cryobiology [Cryobiology] 2024 Jun; Vol. 115, pp. 104902. Date of Electronic Publication: 2024 May 17. - Publication Year :
- 2024
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Abstract
- In this clinical study, we investigated the potential of melatonin (MT) supplementation in the freeze-thaw medium used for cryopreserved human oocytes. In total, 152 patients who underwent in vitro fertilization between January 2020 and December 2022 were included and categorized into different groups as follows: the donor group, comprising 108 patients who donated their oocytes, with 34 patients using a vitrification and warming medium supplemented with MT (D-MT subgroup) and 74 patients using conventional medium without MT (D-0 subgroup); and the autologous group, comprising 38 patients who used their own oocytes, with 19 patients using medium supplemented with MT (A-MT subgroup) and 19 patients using medium without MT (A-0 subgroup). After thawing, the surviving oocytes in the D-MT and A-MT subgroups and D-0 and A-0 subgroups were cultured in a fertilization media with and without 10 <superscript>-9</superscript>  MMT for 2.5 h, respectively, followed by intracytoplasmic sperm injection insemination, embryo culture, and transfer. The survival, cleavage, high-quality embryo, clinical pregnancy, ongoing pregnancy, and implantation rates were significantly higher in the D-MT subgroup than in the D-0 subgroup (all P < 0.05). Similarly, the survival, fertilization, high-quality embryo, and high-quality blastocyst rates were significantly higher in the A-MT subgroup than in the A-0 subgroup (all P < 0.05). These findings indicate that MT addition during cryopreservation can enhance the development of vitrified-warmed human oocytes and improve clinical outcomes.<br />Competing Interests: Declaration of competing interest The authors declare no conflict of interest.<br /> (Copyright © 2024 Society for Cryobiology. Published by Elsevier Inc. All rights reserved.)
- Subjects :
- Humans
Female
Adult
Pregnancy
Pregnancy Rate
Fertilization in Vitro methods
Sperm Injections, Intracytoplasmic methods
Cryoprotective Agents pharmacology
Embryo Transfer
Embryo Culture Techniques methods
Blastocyst drug effects
Melatonin pharmacology
Cryopreservation methods
Oocytes drug effects
Vitrification drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1090-2392
- Volume :
- 115
- Database :
- MEDLINE
- Journal :
- Cryobiology
- Publication Type :
- Academic Journal
- Accession number :
- 38734365
- Full Text :
- https://doi.org/10.1016/j.cryobiol.2024.104902