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Enrichment and Evaluation of Antitumor Properties of Total Flavonoids from Juglans mandshurica Maxim.
- Source :
-
Molecules (Basel, Switzerland) [Molecules] 2024 Apr 25; Vol. 29 (9). Date of Electronic Publication: 2024 Apr 25. - Publication Year :
- 2024
-
Abstract
- Flavonoids are important secondary metabolites found in Juglans mandshurica Maxim., which is a precious reservoir of bioactive substances in China. To explore the antitumor actions of flavonoids (JMFs) from the waste branches of J. mandshurica , the following optimized purification parameters of JMFs by macroporous resins were first obtained. The loading concentration, flow rate, and loading volume of raw flavonoid extracts were 1.4 mg/mL, 2.4 BV/h, and 5 BV, respectively, and for desorption, 60% ethanol (4 BV) was selected to elute JMFs-loaded AB-8 resin at a flow rate of 2.4 BV/h. This adsorption behavior can be explained by the pseudo-second-order kinetic model and Langmuir isotherm model. Subsequently, JMFs were identified using Fourier transform infrared combined with high-performance liquid chromatography and tandem mass spectrometry, and a total of 156 flavonoids were identified. Furthermore, the inhibitory potential of JMFs on the proliferation, migration, and invasion of HepG2 cells was demonstrated. The results also show that exposure to JMFs induced apoptotic cell death, which might be associated with extrinsic and intrinsic pathways. Additionally, flow cytometry detection found that JMFs exposure triggered S phase arrest and the generation of reactive oxygen species in HepG2 cells. These findings suggest that the JMFs purified in this study represent great potential for the treatment of liver cancer.
- Subjects :
- Humans
Hep G2 Cells
Antineoplastic Agents, Phytogenic pharmacology
Antineoplastic Agents, Phytogenic chemistry
Antineoplastic Agents, Phytogenic isolation & purification
Plant Extracts chemistry
Plant Extracts pharmacology
Cell Movement drug effects
Chromatography, High Pressure Liquid
Antineoplastic Agents pharmacology
Antineoplastic Agents chemistry
Juglans chemistry
Flavonoids pharmacology
Flavonoids chemistry
Flavonoids isolation & purification
Cell Proliferation drug effects
Apoptosis drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1420-3049
- Volume :
- 29
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Molecules (Basel, Switzerland)
- Publication Type :
- Academic Journal
- Accession number :
- 38731467
- Full Text :
- https://doi.org/10.3390/molecules29091976