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Improved biodistribution and enhanced immune response of subunit vaccine using a nanostructure formed by self-assembly of ascorbyl palmitate.
- Source :
-
Nanomedicine : nanotechnology, biology, and medicine [Nanomedicine] 2024 Jun; Vol. 58, pp. 102749. Date of Electronic Publication: 2024 May 06. - Publication Year :
- 2024
-
Abstract
- New adjuvant strategies are needed to improve protein-based subunit vaccine immunogenicity. We examined the potential to use nanostructure of 6-O-ascorbyl palmitate to formulate ovalbumin (OVA) protein and an oligodeoxynucleotide (CpG-ODN) (OCC). In mice immunized with a single dose, OCC elicited an OVA-specific immune response superior to OVA/CpG-ODN solution (OC). Rheological studies demonstrated OCC's self-assembling viscoelastic properties. Biodistribution studies indicated that OCC prolonged OVA and CpG-ODN retention at injection site and lymph nodes, reducing systemic spread. Flow-cytometry assays demonstrated that OCC promoted OVA and CpG-ODN co-uptake by Ly6C <superscript>hi</superscript> CD11b <superscript>hi</superscript> CD11c+ monocytes. OCC and OC induced early IFN-γ in lymph nodes, but OCC led to higher concentration. Conversely, mice immunized with OC showed higher serum IFN-γ concentration compared to those immunized with OCC. In mice immunized with OCC, NK1.1+ cells were the IFN-γ major producers, and IFN-γ was essential for OVA-specific IgG2c switching. These findings illustrate how this nanostructure improves vaccine's response.<br />Competing Interests: Declaration of competing interest All authors declared that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.<br /> (Copyright © 2024 Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Mice
Female
Mice, Inbred C57BL
Adjuvants, Immunologic chemistry
Adjuvants, Immunologic pharmacokinetics
Interferon-gamma metabolism
Tissue Distribution
Ascorbic Acid analogs & derivatives
Nanostructures chemistry
Vaccines, Subunit immunology
Vaccines, Subunit chemistry
Vaccines, Subunit pharmacokinetics
Oligodeoxyribonucleotides chemistry
Oligodeoxyribonucleotides pharmacokinetics
Ovalbumin immunology
Ovalbumin chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1549-9642
- Volume :
- 58
- Database :
- MEDLINE
- Journal :
- Nanomedicine : nanotechnology, biology, and medicine
- Publication Type :
- Academic Journal
- Accession number :
- 38719107
- Full Text :
- https://doi.org/10.1016/j.nano.2024.102749