Back to Search
Start Over
Comparison of epidermal growth factor expression and secretion in human salivary glands.
- Source :
-
Archives of oral biology [Arch Oral Biol] 2024 Aug; Vol. 164, pp. 105989. Date of Electronic Publication: 2024 May 04. - Publication Year :
- 2024
-
Abstract
- Objective: To investigate the expression and secretion of epidermal growth factor (EGF) in major and minor salivary gland tissues of human subjects and to examine the potential influence of sex and age on EGF expression and secretion.<br />Design: Saliva samples from the oral cavity at rest and after citric acid stimulation, as well as serum samples, were collected from 150 healthy subjects, and the concentrations of EGF were measured with enzyme-linked immunosorbent assay (ELISA) and compared. The expression of EGF mRNA and protein in normal salivary gland tissues was measured by real-time polymerase chain reaction (RT-PCR), Western blot (WB), and immunohistochemistry (IHC).<br />Results: The EGF concentration in acid-stimulated saliva was significantly higher than that in resting saliva (P < 0.001), and significantly higher than that in serum (P < 0.001). No sex difference was observed in EGF levels of whole saliva and serum, whereas the EGF levels in saliva and serum were decreased with age (P < 0.001 and P < 0.001, respectively). The EGF concentration and compound secretion rate (CSR) in resting submandibular glands saliva were significantly higher than those in resting parotid glands saliva (P = 0.002 and P < 0.001, respectively). The EGF was expressed in all major and minor salivary glands and ranked in order of submandibular, parotid, sublingual, and labial glands.<br />Conclusion: All salivary glands have the function of secreting EGF, and the submandibular gland is the main source of salivary EGF. Aging is a factor influencing the expression and secretion of EGF.<br />Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that influence the work reported in this paper.<br /> (Copyright © 2024 Elsevier Ltd. All rights reserved.)
- Subjects :
- Humans
Female
Male
Adult
Middle Aged
Sex Factors
Aged
Age Factors
RNA, Messenger metabolism
Adolescent
Submandibular Gland metabolism
Salivary Glands, Minor metabolism
Citric Acid metabolism
Epidermal Growth Factor metabolism
Saliva metabolism
Saliva chemistry
Salivary Glands metabolism
Enzyme-Linked Immunosorbent Assay
Blotting, Western
Real-Time Polymerase Chain Reaction
Immunohistochemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1879-1506
- Volume :
- 164
- Database :
- MEDLINE
- Journal :
- Archives of oral biology
- Publication Type :
- Academic Journal
- Accession number :
- 38718466
- Full Text :
- https://doi.org/10.1016/j.archoralbio.2024.105989