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[ Shenqi Chongcao Formula ameliorates inflammatory response in rats with pulmonary fibrosis by activating the ASS1/src/STAT3 signaling pathway].
- Source :
-
Nan fang yi ke da xue xue bao = Journal of Southern Medical University [Nan Fang Yi Ke Da Xue Xue Bao] 2024 Apr 20; Vol. 44 (4), pp. 644-651. - Publication Year :
- 2024
-
Abstract
- Objective: To observe the effect of Shenqi Chongcao (SQCC) Formula on the ASS1/src/STAT3 signaling pathway in a rat model of lung fibrosis and explore its therapeutic mechanism.<br />Methods: A total of 120 male SD rats were divided equally into 5 groups, including a blank control group with saline treatment and 4 groups of rat models of idiopathic pulmonary fibrosis induced by intratracheal instillation of bleomycin. One day after modeling, the rat models were treated with daily gavage of 10 mL/kg saline, SQCC decoction (0.423 g/kg), pirfenidone (10 mL/kg), or intraperitoneal injection of arginine deiminase (ADI; 2.25 mg/kg, every 3 days) for 28 days. After the treatments, the lung tissues of the rats were collected for calculating the lung/body weight ratio, observing histopathology using HE and Masson staining, and analyzing the inflammatory cells in BALF using Giemsa staining. Serum chemokine ligand 2 (CCL2) and transforming growth factor-β1 (TGF-β1) levels were measured with ELISA. The protein expressions of src, p-src <superscript>Try529</superscript> , STAT3, and p-STAT3 <superscript>Try705</superscript> and the mRNA expressions of ASS1, src and STAT3 in the lung tissues were detected using Western blotting and RT-qPCR.<br />Results: The neutrophil, macrophage and lymphocyte counts and serum levels of CCL2 and TGF-β1 were significantly lower in SQCC, pirfenidone and ADI treatment groups than in the model group at each time point of measurement ( P < 0.05). P-src <superscript>Try529</superscript> and p-STAT3 <superscript>Try705</superscript> protein expression levels and ASS1, src, and STAT3 mRNA in the lung tissues were also significantly lower in the 3 treatment groups than in the model group ( P < 0.05).<br />Conclusion: SQCC Formula can alleviate lung fibrosis in rats possibly by activating the ASS1/src/STAT3 signaling pathway in the lung tissues.
- Subjects :
- Animals
Male
Rats
Bleomycin
Chemokine CCL2 metabolism
Disease Models, Animal
Inflammation metabolism
Inflammation drug therapy
Lung metabolism
Lung pathology
Lung drug effects
Rats, Sprague-Dawley
src-Family Kinases drug effects
src-Family Kinases metabolism
Transforming Growth Factor beta1 metabolism
Carbon-Carbon Ligases drug effects
Carbon-Carbon Ligases metabolism
Drugs, Chinese Herbal therapeutic use
Drugs, Chinese Herbal pharmacology
Pulmonary Fibrosis chemically induced
Pulmonary Fibrosis drug therapy
Pulmonary Fibrosis metabolism
Signal Transduction drug effects
STAT3 Transcription Factor drug effects
STAT3 Transcription Factor metabolism
Subjects
Details
- Language :
- Chinese
- ISSN :
- 1673-4254
- Volume :
- 44
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Nan fang yi ke da xue xue bao = Journal of Southern Medical University
- Publication Type :
- Academic Journal
- Accession number :
- 38708496
- Full Text :
- https://doi.org/10.12122/j.issn.1673-4254.2024.04.04