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Identification and functional characterization of a superoxide dismutase (CuZnSOD) from Pinctada fucata martensii.
- Source :
-
Fish & shellfish immunology [Fish Shellfish Immunol] 2024 Jun; Vol. 149, pp. 109599. Date of Electronic Publication: 2024 May 01. - Publication Year :
- 2024
-
Abstract
- Copper/zinc superoxide dismutase (Cu/Zn-SOD) can effectively eliminate reactive oxygen species (ROS),avoid damage from O <subscript>2</subscript> to the body, and maintain O <subscript>2</subscript> balance. In this study, multi-step high-performance liquid chromatography (HPLC), combined with Mass Spectrometry (MS), was used to isolate and identify Cu/Zn-SOD from the serum of Pinctada fucata martensii (P. f. martensii) and was designated as PmECSOD. With a length of 1864 bp and an open reading frame (ORF) of 1422 bp, the cDNA encodes a 473 amino acid protein. The PmECSOD transcript was detected in multiple tissues by quantitative real-time PCR (qRT-PCR), with its highest expression level being in the gills. Additionally, the temporal expression of PmECSOD mRNA in the hemolymph was highest at 48 h after in vivo stimulation with Escherichia coli and Micrococcus luteus. The results from this study provide a valuable base for further exploration of molluscan innate immunity and immune response.<br /> (Copyright © 2024 Elsevier Ltd. All rights reserved.)
- Subjects :
- Animals
Gene Expression Profiling veterinary
Base Sequence
Sequence Alignment veterinary
Escherichia coli
DNA, Complementary genetics
Micrococcus luteus physiology
Gene Expression Regulation immunology
RNA, Messenger genetics
RNA, Messenger metabolism
Pinctada immunology
Pinctada genetics
Pinctada enzymology
Superoxide Dismutase genetics
Superoxide Dismutase chemistry
Superoxide Dismutase metabolism
Superoxide Dismutase immunology
Amino Acid Sequence
Immunity, Innate genetics
Phylogeny
Subjects
Details
- Language :
- English
- ISSN :
- 1095-9947
- Volume :
- 149
- Database :
- MEDLINE
- Journal :
- Fish & shellfish immunology
- Publication Type :
- Academic Journal
- Accession number :
- 38701990
- Full Text :
- https://doi.org/10.1016/j.fsi.2024.109599