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Dual template (epitope) imprinted electrode for sensing bacterial protein with high selectivity.
- Source :
-
Journal of molecular recognition : JMR [J Mol Recognit] 2024 Jul; Vol. 37 (4), pp. e3087. Date of Electronic Publication: 2024 Apr 30. - Publication Year :
- 2024
-
Abstract
- Epitope imprinting has shown better prospects to synthesize synthetic receptors for proteins. Here, dual epitope imprinted polymer electrode (DEIP) matrix was fabricated on gold surface of electrochemical quartz crystal microbalance (EQCM) for recognition of target epitope sequence in blood samples of patients suffering from brain fever. Epitope sequences from outer membrane protein Por B of Neisseria meningitidis (MC58) bacteria predicted through immunoinformatic tools were chosen for imprinting. Self-assembled monolayers (SAM) of cysteine appended epitope sequences on gold nanoparticles were subjected to polymerization prior to electrodeposition on gold coated EQCM electrode. The polymeric matrix was woven around the cysteine appended epitope SAMs through multiple monomers (3-sulfo propyl methacrylate potassium salt (3-SPMAP), benzyl methacrylate (BMA)) and crosslinker (N, N'-methylene-bis-acrylamide). On extraction of the peptide sequences, imprinted cavities were able to selectively and specifically bind targeted epitope sequences in laboratory samples as well as 'real' samples of patients. Selectivity of sensor was examined through mismatched peptide sequences and certain plasma proteins also. The sensor was able to show specific binding towards the blood samples of infected patients, even in the presence of 'matrix' and other plasma proteins such as albumin and globulin. Even other peptide sequences, similar to epitope sequences only with one or two amino acid mismatches were also unable to show any binding. The analytical performance of DEIP-EQCM sensor was tested through selectivity, specificity, matrix effect, detection limit (0.68-1.01 nM), quantification limit (2.05-3.05 nM) and reproducibility (RSD ~ 5%). Hence, a diagnostic tool for bacterium causing meningitis is successfully fabricated in a facile manner which will broaden the clinical access and make efficient population screening feasible.<br /> (© 2024 John Wiley & Sons Ltd.)
- Subjects :
- Humans
Biosensing Techniques methods
Bacterial Proteins chemistry
Bacterial Proteins immunology
Metal Nanoparticles chemistry
Porins chemistry
Porins immunology
Epitopes immunology
Epitopes chemistry
Electrodes
Neisseria meningitidis immunology
Gold chemistry
Molecular Imprinting
Quartz Crystal Microbalance Techniques
Subjects
Details
- Language :
- English
- ISSN :
- 1099-1352
- Volume :
- 37
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Journal of molecular recognition : JMR
- Publication Type :
- Academic Journal
- Accession number :
- 38686731
- Full Text :
- https://doi.org/10.1002/jmr.3087