Back to Search
Start Over
The Epstein-Barr virus-miRNA-BART6-5p regulates TGF-β/SMAD4 pathway to induce glycolysis and enhance proliferation and metastasis of gastric cancer cells.
- Source :
-
Oncology research [Oncol Res] 2024 Apr 23; Vol. 32 (5), pp. 999-1009. Date of Electronic Publication: 2024 Apr 23 (Print Publication: 2024). - Publication Year :
- 2024
-
Abstract
- Background: EBV-miR-BARTs exhibit significant relevance in epithelial tumors, particularly in EBV-associated gastric and nasopharyngeal cancers. However, their specific mechanisms in the initiation and progression of gastric cancer remain insufficiently explored.<br />Material and Methods: Initially, EBV-miRNA-BART6-5p and its target gene SMAD4 expression were assessed in EBV-associated gastric cancer tissues and cell lines. Subsequent transfection induced overexpression of EBV-miRNA-BART6-5p in AGS and MKN-45, and downregulation in EBV-positive cells (SUN-719). The subsequent evaluation aimed to observe their impact on gastric cancer cell proliferation, migration, and glycolytic processes, with the TGF-β/SMAD4 signaling pathway value clarified using a TGF-β inhibitor.<br />Results: EBV-miRNA-BART6-5p exhibits pronounced upregulation in EBV-associated gastric cancer tissues and EBV-positive cells, while its target gene SMAD4 demonstrates downregulated expression. Upregulation of it can promote the proliferation and migration of gastric cancer cells. Additionally, We found EBV-miRNA-BART6-5p promotes glycolysis of gastric cancer cells. Inhibition of the TGF-β/SMAD4 signaling pathway resulted in suppressed proliferation and migration of gastric cancer cells, concomitant with a diminished glycolytic capacity.<br />Conclusion: In this study, we found that EBV-miRNA-BART6-5p can target SMAD4, effectively increasing glycolysis in gastric cancer cells by regulating the TGF-β/SMAD4 signaling pathway, thereby enhancing the proliferation and metastasis of gastric cancer cells. Our findings may offer new insights into the metabolic aspects of gastric cancer.<br />Competing Interests: The authors declare that they have no conflicts of interest to report regarding the present study.<br /> (© 2024 Zhao et al.)
- Subjects :
- Humans
Cell Line, Tumor
Epstein-Barr Virus Infections genetics
Epstein-Barr Virus Infections virology
Epstein-Barr Virus Infections metabolism
Epstein-Barr Virus Infections complications
Epstein-Barr Virus Infections pathology
Neoplasm Metastasis
RNA, Viral genetics
Stomach Neoplasms pathology
Stomach Neoplasms virology
Stomach Neoplasms genetics
Stomach Neoplasms metabolism
Smad4 Protein genetics
Smad4 Protein metabolism
MicroRNAs genetics
Cell Proliferation
Glycolysis genetics
Transforming Growth Factor beta metabolism
Transforming Growth Factor beta genetics
Herpesvirus 4, Human genetics
Signal Transduction
Cell Movement genetics
Gene Expression Regulation, Neoplastic
Subjects
Details
- Language :
- English
- ISSN :
- 1555-3906
- Volume :
- 32
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Oncology research
- Publication Type :
- Academic Journal
- Accession number :
- 38686046
- Full Text :
- https://doi.org/10.32604/or.2024.046679