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Design and evaluation of nanostructured lipid carrier of Bergenin isolated from Pentaclethra macrophylla for anti-inflammatory effect on lipopolysaccharide-induced inflammatory responses in macrophages.
- Source :
-
European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V [Eur J Pharm Biopharm] 2024 Jul; Vol. 200, pp. 114307. Date of Electronic Publication: 2024 Apr 27. - Publication Year :
- 2024
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Abstract
- Herein, we report the properties of nanostructured lipid carriers (NLCs) prepared with a gradient concentration of Bergenin (BGN) isolated from Pentaclethra macrophylla stem bark powder. A gradient concentration of BGN (BGN 0, 50, 100, 150, and 200 mg) was prepared in a 5 % lipid matrix consisting of Transcutol HP (75 %), Phospholipon 90H (15 %), and Gelucire 43/01 (10 %) to which a surfactant aqueous phase consisting of Tween 80, sorbitol, and sorbic acid was dissolved. The NLCs were evaluated by size, polydispersity index (PDI), zeta potential, Fourier Transform Infrared Spectroscopy (FTIR), Differential Scanning Calorimetry (DSC), encapsulation efficiency, and in vitro drug release. The result shows polydispersed nanoparticles with high drug encapsulation (94.26-99.50 %). The nanoparticles were mostly spherical, but those from the 50 mg BGN batch were more cuboidal than spherical. The drug release was highest from the latter to the tune of 40 % compared to the pure BGN solution, which released about 15 % BGN. The anti-inflammatory activity of the BGN-NLC and total plant extract was studied on lipopolysaccharide (LPS)-inflamed macrophages. The cell study showed that BGN and plant extract had low cytotoxicity on macrophages and exhibited a dose-dependent anti-inflammatory effect on the LPS-induced inflammatory process in macrophages.<br />Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.<br /> (Copyright © 2024. Published by Elsevier B.V.)
- Subjects :
- Animals
Mice
RAW 264.7 Cells
Drug Liberation
Nanostructures chemistry
Saxifragaceae chemistry
Particle Size
Inflammation drug therapy
Inflammation chemically induced
Plant Extracts pharmacology
Plant Extracts administration & dosage
Plant Extracts chemistry
Lipopolysaccharides pharmacology
Macrophages drug effects
Macrophages metabolism
Anti-Inflammatory Agents pharmacology
Anti-Inflammatory Agents administration & dosage
Anti-Inflammatory Agents chemistry
Drug Carriers chemistry
Benzopyrans pharmacology
Benzopyrans administration & dosage
Benzopyrans chemistry
Nanoparticles chemistry
Lipids chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1873-3441
- Volume :
- 200
- Database :
- MEDLINE
- Journal :
- European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V
- Publication Type :
- Academic Journal
- Accession number :
- 38685438
- Full Text :
- https://doi.org/10.1016/j.ejpb.2024.114307