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Construction of an immunosensor based on Cys/Au@TiO 2 modification for the detection of liver cancer marker PIVKA-II.
- Source :
-
Talanta [Talanta] 2024 Aug 01; Vol. 275, pp. 126082. Date of Electronic Publication: 2024 Apr 16. - Publication Year :
- 2024
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Abstract
- An ultrasensitive immunosensor of Cys/Au@TiO <subscript>2</subscript> based on disposable screen-printed electrodes (SPE) for PIVKA-II detection for hepatocellular carcinoma (HCC) diagnosis was developed by utilizing Cystine (Cys) and nanocomposite Au@TiO <subscript>2</subscript> . Firstly, HAuCl <subscript>4</subscript> underwent a reduction reaction with NaBH <subscript>4</subscript> , then Au nanoparticles were coated onto TiO <subscript>2</subscript> nanoparticles. Followed, Cys/Au@TiO <subscript>2</subscript> was formed through self-assembly of cysteine to allow the monoclonal antibody of abnormal thrombospondin to bound to the amino group on the surface of the composite by covalent bonding. The mechanism is to determine the changes in the current of the sensor caused by the specific binding of the abnormal prothrombin monoclonal antibody adsorbed by the complex with its antigen. The Cys/Au@TiO <subscript>2</subscript> immunosensor was fully characterized by various analytical approaches and it showed a wide linear testing range of 1-10000 pg mL <superscript>-1</superscript> (R <superscript>2</superscript>  = 0.991) and the limit of detection down to 0.77 pg ml <superscript>-1</superscript> , with highly sensitivity and specificity. The results showed that the developed immunosensor platform can effectively detect trace amounts of PIVKA-II protein and has potent clinical application for HCC diagnosis.<br />Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.<br /> (Copyright © 2024. Published by Elsevier B.V.)
- Subjects :
- Humans
Immunoassay methods
Metal Nanoparticles chemistry
Limit of Detection
Electrodes
Carcinoma, Hepatocellular diagnosis
Protein Precursors
Biomarkers
Titanium chemistry
Gold chemistry
Liver Neoplasms diagnosis
Biomarkers, Tumor analysis
Biomarkers, Tumor immunology
Prothrombin chemistry
Biosensing Techniques methods
Cysteine chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1873-3573
- Volume :
- 275
- Database :
- MEDLINE
- Journal :
- Talanta
- Publication Type :
- Academic Journal
- Accession number :
- 38677167
- Full Text :
- https://doi.org/10.1016/j.talanta.2024.126082