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Chip-based automated equipment for dual-mode point-of-care testing foodborne pathogens.
- Source :
-
Biosensors & bioelectronics [Biosens Bioelectron] 2024 Aug 01; Vol. 257, pp. 116338. Date of Electronic Publication: 2024 Apr 25. - Publication Year :
- 2024
-
Abstract
- Foodborne pathogens have a substantial bearing on food safety and environmental health. The development of automated, portable and compact devices is essential for the on-site and rapid point-of-care testing (POCT) of bacteria. Here, this work developed a micro-automated microfluidic device for detecting bacteria, such as Escherichia coli (E. coli) O157:H7, using a seashell-like microfluidic chip (SMC) as an analysis and mixing platform. The automated device integrates a colorimetric/fluorescent system for the metabolism of copper (Cu <superscript>2+</superscript> ) by E. coli affecting o-phenylenediamine (OPD) for concentration analysis. A smartphone was used to read the RGB data of the chip reaction reservoir to detect colorimetric and fluorescence patterns in the concentration range of 10 <superscript>2</superscript> -10 <superscript>6</superscript>  CFU mL <superscript>-1</superscript> . The automated device overcomes the low efficiency and tedious steps of traditional detection and enables high-precision automated detection that can be applied to POCT in the field, providing an ideal solution for broadening the application of E. coli detection.<br />Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.<br /> (Copyright © 2024 Elsevier B.V. All rights reserved.)
- Subjects :
- Humans
Smartphone instrumentation
Foodborne Diseases microbiology
Phenylenediamines chemistry
Escherichia coli Infections microbiology
Food Contamination analysis
Biosensing Techniques instrumentation
Biosensing Techniques methods
Escherichia coli O157 isolation & purification
Lab-On-A-Chip Devices
Point-of-Care Testing
Food Microbiology
Colorimetry instrumentation
Copper chemistry
Equipment Design
Subjects
Details
- Language :
- English
- ISSN :
- 1873-4235
- Volume :
- 257
- Database :
- MEDLINE
- Journal :
- Biosensors & bioelectronics
- Publication Type :
- Academic Journal
- Accession number :
- 38677017
- Full Text :
- https://doi.org/10.1016/j.bios.2024.116338