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Immune-enhancing activity of compound polysaccharide on the inactivated influenza vaccine.
- Source :
-
Carbohydrate polymers [Carbohydr Polym] 2024 Jul 15; Vol. 336, pp. 122080. Date of Electronic Publication: 2024 Mar 26. - Publication Year :
- 2024
-
Abstract
- Traditional Chinese medicine polysaccharides have numerous biological activities with broad applications in the biomedical industries. However, a clear understanding of the pharmacological activities of compound polysaccharides with multi-component structures remain challenging. This study aimed to investigate the immune boosting effect of compound polysaccharides on the influenza vaccine and assess the preliminary structure-activity relationship. The compound polysaccharide (CP) was isolated from the combined Chinese herbs lentinan, pachymaran and tremellan, and purified by gradient ethanol precipitation to obtain its subcomponents of CP-20, CP-40, CP-60, and CP-80 with decreasing molecular weights. These polysaccharides were mainly composed of glucans with different linkage patterns, including α-(1 → 3)-glucan, α-(1 → 4)-glucan and β-(1 → 6)-glucan. A significant improvement was observed in the survival of mice vaccinated with inactivated (IAV) vaccine and the isolated polysaccharides as adjuvants. A reduction in the pulmonary virus titer and weight loss were also observed. Moreover, CP-40 and CP-60, as well as the original CP, significantly enhanced the serum anti-IAV antibody titers and interleukin IL-2, IL-5, and IL-6 concentrations. These preliminary results indicate the immune boosting effect of the compound polysaccharides is highly relevant to the specific structural properties of the subcomponent, and CP-40 is worthy of further exploration as a glycan adjuvant for the IAV vaccine.<br />Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.<br /> (Copyright © 2024 Elsevier Ltd. All rights reserved.)
- Subjects :
- Animals
Mice
Female
Antibodies, Viral blood
Antibodies, Viral immunology
Orthomyxoviridae Infections prevention & control
Orthomyxoviridae Infections immunology
Cytokines metabolism
Influenza Vaccines immunology
Influenza Vaccines chemistry
Influenza Vaccines pharmacology
Vaccines, Inactivated immunology
Polysaccharides pharmacology
Polysaccharides chemistry
Polysaccharides isolation & purification
Adjuvants, Immunologic pharmacology
Adjuvants, Immunologic chemistry
Mice, Inbred BALB C
Subjects
Details
- Language :
- English
- ISSN :
- 1879-1344
- Volume :
- 336
- Database :
- MEDLINE
- Journal :
- Carbohydrate polymers
- Publication Type :
- Academic Journal
- Accession number :
- 38670772
- Full Text :
- https://doi.org/10.1016/j.carbpol.2024.122080