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An Extracellular Matrix Overlay Model for Bioluminescence Microscopy to Measure Single-Cell Heterogeneous Responses to Antiandrogens in Prostate Cancer Cells.

Authors :
Champagne A
Chebra I
Jain P
Ringuette Goulet C
Lauzier A
Guyon A
Neveu B
Pouliot F
Source :
Biosensors [Biosensors (Basel)] 2024 Apr 05; Vol. 14 (4). Date of Electronic Publication: 2024 Apr 05.
Publication Year :
2024

Abstract

Prostate cancer (PCa) displays diverse intra-tumoral traits, impacting its progression and treatment outcomes. This study aimed to refine PCa cell culture conditions for dynamic monitoring of androgen receptor (AR) activity at the single-cell level. We introduced an extracellular matrix-Matrigel (ECM-M) culture model, enhancing cellular tracking during bioluminescence single-cell imaging while improving cell viability. ECM-M notably tripled the traceability of poorly adherent PCa cells, facilitating robust single-cell tracking, without impeding substrate permeability or AR response. This model effectively monitored AR modulation by antiandrogens across various PCa cell lines. Single-cell imaging unveiled heterogeneous antiandrogen responses within populations, correlating non-responsive cell proportions with drug IC50 values. Integrating ECM-M culture with the PSEBC-TSTA biosensor enabled precise characterization of ARi responsiveness within diverse cell populations. Our ECM-M model stands as a promising tool to assess heterogeneous single-cell treatment responses in cancer, offering insights to link drug responses to intracellular signaling dynamics. This approach enhances our comprehension of the nuanced and dynamic nature of PCa treatment responses.

Details

Language :
English
ISSN :
2079-6374
Volume :
14
Issue :
4
Database :
MEDLINE
Journal :
Biosensors
Publication Type :
Academic Journal
Accession number :
38667168
Full Text :
https://doi.org/10.3390/bios14040175