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An exploratory study investigating the impact of the bladder tumor microbiome on Bacillus Calmette Guerin (BCG) response in non-muscle invasive bladder cancer.
- Source :
-
Urologic oncology [Urol Oncol] 2024 Sep; Vol. 42 (9), pp. 291.e1-291.e11. Date of Electronic Publication: 2024 Apr 24. - Publication Year :
- 2024
-
Abstract
- Purpose: Intravesical Bacillus Calmette-Guerin (BCG) is standard of care for intermediate- and high-risk non-muscle invasive bladder cancer (NMIBC). The effect of the bladder microbiome on response to BCG is unclear. We sought to characterize the microbiome of bladder tumors in BCG-responders and non-responders and identify potential mechanisms that drive treatment response.<br />Materials and Methods: Patients with archival pre-treatment biopsy samples (2012-2018) were identified retrospectively. Prospectively, urine and fresh tumor samples were collected from individuals with high-risk NMIBC (2020-2023). BCG response was defined as tumor-free 2 years from induction therapy. Extracted DNA was sequenced for 16S rRNA and shotgun metagenomics. Primary outcomes were species richness (α-diversity) and microbial composition (β-diversity). Paired t-tests were performed for α-diversity (Observed species/Margalef). Statistical analysis for β-diversity (weighted and unweighted UniFrac distances, weighted Bray-Curtis dissimilarity) were conducted through Permanova, with 999 permutations.<br />Results: Microbial species richness (P < 0.001) and composition (P = 0.001) differed between BCG responders and non-responders. Lactobacillus spp. were significantly enriched in BCG-responders. Shotgun metagenomics identified possible mechanistic pathways such as assimilatory sulfate reduction.<br />Conclusion: A compositional difference exists in the tumor microbiome of BCG responders and non-responders with Lactobacillus having increased abundance in BCG responders.<br />Competing Interests: Declaration of competing interest We declare that we have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.<br /> (Copyright © 2024 Elsevier Inc. All rights reserved.)
- Subjects :
- Humans
Male
Female
Aged
Retrospective Studies
Middle Aged
Neoplasm Invasiveness
Adjuvants, Immunologic therapeutic use
Treatment Outcome
Administration, Intravesical
Non-Muscle Invasive Bladder Neoplasms
Urinary Bladder Neoplasms microbiology
Urinary Bladder Neoplasms pathology
Urinary Bladder Neoplasms drug therapy
BCG Vaccine therapeutic use
Microbiota
Subjects
Details
- Language :
- English
- ISSN :
- 1873-2496
- Volume :
- 42
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Urologic oncology
- Publication Type :
- Academic Journal
- Accession number :
- 38664180
- Full Text :
- https://doi.org/10.1016/j.urolonc.2024.04.011