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Delivery of Stem Cells and BMP-2 With Functionalized Self-Assembling Peptide Enhances Regeneration of Infarcted Myocardium.
- Source :
-
Stem cell reviews and reports [Stem Cell Rev Rep] 2024 Aug; Vol. 20 (6), pp. 1540-1554. Date of Electronic Publication: 2024 Apr 24. - Publication Year :
- 2024
-
Abstract
- Stem cell transplantation is a promising therapeutic strategy for myocardial infarction (MI). However, engraftment, survival and differentiation of the transplanted stem cells in ischemic and inflammatory microenvironment are poor. We designed a novel self-assembly peptide (SAP) by modifying the peptide RADA16 with cell-adhesive motif and BMP-2 (bone morphogenetic protein-2)-binding motif. Effects of the functionalized SAP on adhesion, survival and differentiation of c-kit <superscript>+</superscript> MSCs (mesenchymal stem cells) were examined. Myocardial regeneration, neovascularization and cardiac function were assessed after transplantation of the SAP loading c-kit <superscript>+</superscript> MSCs and BMP-2 in rat MI models. The SAP could spontaneously assemble into well-ordered nanofibrous scaffolds. The cells adhered to the SAP scaffolds and spread well. The SAP protected the cells in the condition of hypoxia and serum deprivation. Following degradation of the SAP, BMP-2 was released sustainedly and induced c-kit <superscript>+</superscript> MSCs to differentiate into cardiomyocytes. At four weeks after transplantation of the SAP loading c-kit <superscript>+</superscript> MSCs and BMP-2, myocardial regeneration and angiogenesis were enhanced, and cardiac function was improved significantly. The cardiomyocytes differentiated from the engrafted c-kit <superscript>+</superscript> MSCs were increased markedly. The differentiated cells connected with recipient cardiomyocytes to form gap junctions. Collagen volume was decreased dramatically. These results suggest that the functionalized SAP promotes engraftment, survival and differentiation of stem cells effectively. Local sustained release of BMP-2 with SAP is a viable strategy to enhance differentiation of the engrafted stem cells and repair of the infarcted myocardium.<br /> (© 2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
- Subjects :
- Animals
Rats
Myocytes, Cardiac drug effects
Myocytes, Cardiac metabolism
Myocytes, Cardiac cytology
Male
Rats, Sprague-Dawley
Neovascularization, Physiologic drug effects
Myocardium pathology
Myocardium metabolism
Tissue Scaffolds chemistry
Nanofibers chemistry
Proto-Oncogene Proteins c-kit metabolism
Cell Adhesion drug effects
Myocardial Infarction therapy
Myocardial Infarction pathology
Bone Morphogenetic Protein 2 pharmacology
Bone Morphogenetic Protein 2 metabolism
Cell Differentiation drug effects
Mesenchymal Stem Cells metabolism
Mesenchymal Stem Cells cytology
Mesenchymal Stem Cells drug effects
Regeneration drug effects
Mesenchymal Stem Cell Transplantation
Peptides pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 2629-3277
- Volume :
- 20
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Stem cell reviews and reports
- Publication Type :
- Academic Journal
- Accession number :
- 38656478
- Full Text :
- https://doi.org/10.1007/s12015-024-10721-7