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[Carbapenemase Genes, Virulence Genes, and Molecular Epidemiology of Carbapenem-Resistant Klebsiella pneumoniae Derived From Bloodstream Infections].
- Source :
-
Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition [Sichuan Da Xue Xue Bao Yi Xue Ban] 2024 Mar 20; Vol. 55 (2), pp. 391-396. - Publication Year :
- 2024
-
Abstract
- Objective: To investigate the clinical characteristics and molecular epidemiology of carbapenem-resistant Klebsiella pneumoniae (CRKP) isolated from patients with bloodstream infections in a large tertiary-care general hospital in Southwest China.<br />Methods: A total of 131 strains of non-repeating CRKP were collected from the blood cultures of patients who had bloodstream infections in 2015-2019. The strains were identified by VITEK-2, a fully automated microbial analyzer, and matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry. The minimum inhibitory concentration (MIC) was determined by microbroth dilution method. The common carbapenemase resistant genes and virulence factors were identified by PCR. Homology analysis was performed by multilocus sequencing typing. Whole genome sequencing was performed to analyze the genomic characteristics of CRKP without carbapenemase.<br />Results: The 131 strains of CRKP showed resistance to common antibiotics, except for polymyxin B (1.6% resistance rate) and tigacycline (8.0% resistance rate). A total of 105 (80.2%) CRKP strains carried the Klebsiella pneumoniae carbapenemase ( KPC ) resistance gene, 15 (11.4%) strains carried the New Delhi Metallo-β-lactamase ( NDM ) gene, and 4 (3.1%) isolates carried both KPC and NDM genes. Sequence typing (ST) 11 (74.0%) was the dominant sequence type. High detection rates for mrkD (96.2%), fimH (98.5%), entB (100%), and other virulence genes were reported. One hypervirulent CRKP strain was detected. The seven strains of CRKP that did not produce carbapenemase were shown to carry ESBL or AmpC genes and had anomalies in membrane porins OMPK35 and OMPK36, according to whole genome sequencing.<br />Conclusion: In a large-scale tertiary-care general hospital, CRKP mainly carries the KPC gene, has a high drug resistance rate to a variety of antibiotics, and possesses multiple virulence genes. Attention should be paid to CRKP strains with high virulence.<br />Competing Interests: 利益冲突 所有作者均声明不存在利益冲突<br /> (© 2024《四川大学学报(医学版)》编辑部 版权所有Copyright ©2024 Editorial Board of Journal of Sichuan University (Medical Sciences).)
- Subjects :
- Humans
China epidemiology
Anti-Bacterial Agents pharmacology
Virulence genetics
Male
Female
Carbapenem-Resistant Enterobacteriaceae genetics
Carbapenem-Resistant Enterobacteriaceae isolation & purification
Carbapenem-Resistant Enterobacteriaceae drug effects
Middle Aged
Bacteremia microbiology
Bacteremia epidemiology
Whole Genome Sequencing methods
Klebsiella pneumoniae genetics
Klebsiella pneumoniae drug effects
Klebsiella pneumoniae isolation & purification
Klebsiella pneumoniae pathogenicity
Bacterial Proteins genetics
beta-Lactamases genetics
Klebsiella Infections microbiology
Klebsiella Infections epidemiology
Carbapenems pharmacology
Microbial Sensitivity Tests
Virulence Factors genetics
Molecular Epidemiology
Subjects
Details
- Language :
- Chinese
- ISSN :
- 1672-173X
- Volume :
- 55
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition
- Publication Type :
- Academic Journal
- Accession number :
- 38645859
- Full Text :
- https://doi.org/10.12182/20240360202