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Rapid detection of plasmid-mediated AmpC-producers by eazyplex® SuperBug AmpC assay compared to whole-genome sequencing.
- Source :
-
Journal of microbiological methods [J Microbiol Methods] 2024 Jun; Vol. 221, pp. 106938. Date of Electronic Publication: 2024 Apr 19. - Publication Year :
- 2024
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Abstract
- Current methods for plasmid-mediated AmpC β-lactamase (pAmpC) detection in routine microbiological laboratories are based on various phenotypic tests. Eazyplex®SuperBug AmpC assay is a molecular assay based on isothermal amplification for rapid detection of the most common pAmpC types from bacterial culture: CMY-2 group, DHA, ACC and MOX. Our aim was to evaluate the diagnostic performance of this assay. The assay was evaluated on 64 clinical isolates of Enterobacterales without chromosomal inducible AmpC, and with phenotypically confirmed AmpC production. The results were confirmed, and isolates further characterized by whole-genome sequencing (WGS). eazyplex®SuperBug AmpC assay correctly detected the two most common pAmpC types CMY-2 group (16/16) and DHA (19/19). Detection of ACC and MOX could not be evaluated on our set of isolates since there was only one isolate harbouring ACC and none with MOX. pAmpC encoding genes could be detected in only eight of 36 investigated Escherichia coli isolates. The remaining 28 E. coli isolates harboured previously described mutations in the bla <subscript>EC</subscript> promoter, leading to the overexpression of chromosomally encoded E. coli specific AmpC β-lactamase. All results were 100% concordant with the results of WGS. eazyplex®SuperBug AmpC assay enabled rapid and reliable detection of pAmpC-encoding genes in Enterobacterales like Klebsiella spp. and Proteus spp. and the distinction between plasmid-mediated and chromosomally encoded AmpC in E. coli.<br />Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.<br /> (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)
- Subjects :
- Anti-Bacterial Agents pharmacology
Enterobacteriaceae genetics
Enterobacteriaceae isolation & purification
Enterobacteriaceae enzymology
Escherichia coli genetics
Escherichia coli isolation & purification
Microbial Sensitivity Tests
Nucleic Acid Amplification Techniques methods
Whole Genome Sequencing methods
Bacterial Proteins genetics
beta-Lactamases genetics
Plasmids genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1872-8359
- Volume :
- 221
- Database :
- MEDLINE
- Journal :
- Journal of microbiological methods
- Publication Type :
- Academic Journal
- Accession number :
- 38642781
- Full Text :
- https://doi.org/10.1016/j.mimet.2024.106938