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Mussel shell-derived pro-regenerative scaffold with conductive porous multi-scale-patterned microenvironment for spinal cord injury repair.
- Source :
-
Biomedical materials (Bristol, England) [Biomed Mater] 2024 Apr 30; Vol. 19 (3). Date of Electronic Publication: 2024 Apr 30. - Publication Year :
- 2024
-
Abstract
- It is well-established that multi-scale porous scaffolds can guide axonal growth and facilitate functional restoration after spinal cord injury (SCI). In this study, we developed a novel mussel shell-inspired conductive scaffold for SCI repair with ease of production, multi-scale porous structure, high flexibility, and excellent biocompatibility. By utilizing the reducing properties of polydopamine, non-conductive graphene oxide (GO) was converted into conductive reduced graphene oxide (rGO) and crosslinked in situ within the mussel shells. In vitro experiments confirmed that this multi-scale porous Shell@PDA-GO could serve as structural cues for enhancing cell adhesion, differentiation, and maturation, as well as promoting the electrophysiological development of hippocampal neurons. After transplantation at the injury sites, the Shell@PDA-GO provided a pro-regenerative microenvironment, promoting endogenous neurogenesis, triggering neovascularization, and relieving glial fibrosis formation. Interestingly, the Shell@PDA-GO could induce the release of endogenous growth factors (NGF and NT-3), resulting in the complete regeneration of nerve fibers at 12 weeks. This work provides a feasible strategy for the exploration of conductive multi-scale patterned scaffold to repair SCI.<br /> (© 2024 IOP Publishing Ltd.)
- Subjects :
- Animals
Porosity
Indoles chemistry
Animal Shells chemistry
Cell Differentiation
Electric Conductivity
Neurons
Rats
Rats, Sprague-Dawley
Cell Adhesion
Neurogenesis
Tissue Engineering methods
Nerve Growth Factor metabolism
Nerve Growth Factor chemistry
Hippocampus
Spinal Cord Injuries therapy
Tissue Scaffolds chemistry
Graphite chemistry
Bivalvia
Nerve Regeneration
Polymers chemistry
Biocompatible Materials chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1748-605X
- Volume :
- 19
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Biomedical materials (Bristol, England)
- Publication Type :
- Academic Journal
- Accession number :
- 38626779
- Full Text :
- https://doi.org/10.1088/1748-605X/ad3f63