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Mechanically reinforced hydrogel vehicle delivering angiogenic factor for beta cell therapy.
- Source :
-
Journal of colloid and interface science [J Colloid Interface Sci] 2024 Aug; Vol. 667, pp. 54-63. Date of Electronic Publication: 2024 Apr 09. - Publication Year :
- 2024
-
Abstract
- Type 1 diabetes mellitus (T1DM) is a chronic disease affecting millions worldwide. Insulin therapy is currently the golden standard for treating T1DM; however, it does not restore the normal glycaemic balance entirely, which increases the risk of secondary complications. Beta-cell therapy may be a possible way of curing T1DM and has already shown promising results in the clinic. However, low retention rates, poor cell survival, and limited therapeutic potential are ongoing challenges, thus increasing the need for better cell encapsulation devices. This study aimed to develop a mechanically reinforced vascular endothelial growth factor (VEGF)-delivering encapsulation device suitable for beta cell encapsulation and transplantation. Poly(l-lactide-co-ε-caprolactone) (PLCL)/gelatin methacryloyl (GelMA)/alginate coaxial nanofibres were produced using electrospinning and embedded in an alginate hydrogel. The encapsulation device was physically and biologically characterised and was found to be suitable for INS-1E beta cell encapsulation, vascularization, and transplantation in terms of its biocompatibility, porosity, swelling ratio and mechanical properties. Lastly, VEGF was incorporated into the hydrogel and the release kinetics and functional studies revealed a sustained release of bioactive VEGF for at least 14 days, making the modified alginate system a promising candidate for improving the beta cell survival after transplantation.<br />Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.<br /> (Copyright © 2024. Published by Elsevier Inc.)
- Subjects :
- Animals
Polyesters chemistry
Rats
Cell Survival drug effects
Humans
Diabetes Mellitus, Type 1 therapy
Methacrylates chemistry
Angiogenesis Inducing Agents chemistry
Angiogenesis Inducing Agents pharmacology
Angiogenesis Inducing Agents administration & dosage
Surface Properties
Hydrogels chemistry
Alginates chemistry
Insulin-Secreting Cells metabolism
Insulin-Secreting Cells cytology
Vascular Endothelial Growth Factor A metabolism
Vascular Endothelial Growth Factor A administration & dosage
Gelatin chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1095-7103
- Volume :
- 667
- Database :
- MEDLINE
- Journal :
- Journal of colloid and interface science
- Publication Type :
- Academic Journal
- Accession number :
- 38615623
- Full Text :
- https://doi.org/10.1016/j.jcis.2024.04.050