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68 Ga-NC-BCH Whole-Body PET Imaging Rapidly Targets Claudin18.2 in Lesions in Gastrointestinal Cancer Patients.
- Source :
-
Journal of nuclear medicine : official publication, Society of Nuclear Medicine [J Nucl Med] 2024 Jun 03; Vol. 65 (6), pp. 856-863. Date of Electronic Publication: 2024 Jun 03. - Publication Year :
- 2024
-
Abstract
- <superscript>68</superscript> Ga-labeled nanobody ( <superscript>68</superscript> Ga-NC-BCH) is a single-domain antibody-based PET imaging agent. We conducted a first-in-humans study of <superscript>68</superscript> Ga-NC-BCH for PET to determine its in vivo biodistribution, metabolism, radiation dosimetry, safety, and potential for quantifying claudin-18 isoform 2 (CLDN18.2) expression in gastrointestinal cancer patients. Methods: Initially, we synthesized the probe <superscript>68</superscript> Ga-NC-BCH and performed preclinical evaluations on human gastric adenocarcinoma cell lines and xenograft mouse models. Next, we performed a translational study with a pilot cohort of patients with advanced gastrointestinal cancer on a total-body PET/CT scanner. Radiopharmaceutical biodistribution, radiation dosimetry, and the relationship between tumor uptake and CLDN18.2 expression were evaluated. Results: <superscript>68</superscript> Ga-NC-BCH was stably prepared and demonstrated good radiochemical properties. According to preclinical evaluation, <superscript>68</superscript> Ga-NC-BCH exhibited rapid blood clearance, high affinity for CLDN18.2, and high specific uptake in CLDN18.2-positive cells and xenograft mouse models. <superscript>68</superscript> Ga-NC-BCH displayed high uptake in the stomach and kidney and slight uptake in the pancreas. Compared with <superscript>18</superscript> F-FDG, <superscript>68</superscript> Ga-NC-BCH showed significant differences in uptake in lesions with different levels of CLDN18.2 expression. Conclusion: A clear correlation was detected between PET SUV and CLDN18.2 expression, suggesting that <superscript>68</superscript> Ga-NC-BCH PET could be used as a companion diagnostic tool for optimizing treatments that target CLDN18.2 in tumors.<br /> (© 2024 by the Society of Nuclear Medicine and Molecular Imaging.)
- Subjects :
- Humans
Animals
Mice
Cell Line, Tumor
Female
Male
Tissue Distribution
Middle Aged
Positron-Emission Tomography methods
Positron Emission Tomography Computed Tomography methods
Aged
Radiopharmaceuticals pharmacokinetics
Claudins metabolism
Gastrointestinal Neoplasms diagnostic imaging
Gastrointestinal Neoplasms metabolism
Gallium Radioisotopes
Whole Body Imaging
Subjects
Details
- Language :
- English
- ISSN :
- 1535-5667
- Volume :
- 65
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Journal of nuclear medicine : official publication, Society of Nuclear Medicine
- Publication Type :
- Academic Journal
- Accession number :
- 38604764
- Full Text :
- https://doi.org/10.2967/jnumed.123.267110