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CRISPR/Cas9 gene editing in Drosophila via visual selection in a summer classroom.
- Source :
-
BioRxiv : the preprint server for biology [bioRxiv] 2024 Mar 30. Date of Electronic Publication: 2024 Mar 30. - Publication Year :
- 2024
-
Abstract
- CRISPR/Cas9 methods are a powerful in vivo approach to edit the genome of Drosophila melanogaster . To convert existing Drosophila GAL4 lines to LexA driver lines in a secondary school classroom setting, we applied the CRISPR-based genetic approach to a collection of Gal4 'driver' lines. The integration of the yellow <superscript>+</superscript> coat color marker into homology-assisted CRISPR knock-in (HACK) enabled visual selection of Gal4 -to- LexA conversions using brightfield stereo-microscopy available in a broader set of standard classrooms. Here, we report the successful conversion of eleven Gal4 lines with expression in neuropeptide-expressing cells into corresponding, novel LexA drivers. The conversion was confirmed by LexA - and Gal4 -specific GFP reporter gene expression. This curriculum was successfully implemented in a summer course running 16 hours/week for seven weeks. The modularity, flexibility, and compactness of this course should enable development of similar classes in secondary schools and undergraduate curricula, to provide opportunities for experience-based science instruction, and university-secondary school collaborations that simultaneously fulfill research needs in the community of science.
Details
- Language :
- English
- Database :
- MEDLINE
- Journal :
- BioRxiv : the preprint server for biology
- Accession number :
- 38585736
- Full Text :
- https://doi.org/10.1101/2024.03.28.587232