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A novel aldo-keto reductase gene, OsAKR1, from rice confers higher tolerance to cadmium stress in rice by an in vivo reactive aldehyde detoxification.
- Source :
-
Journal of hazardous materials [J Hazard Mater] 2024 May 15; Vol. 470, pp. 134212. Date of Electronic Publication: 2024 Apr 03. - Publication Year :
- 2024
-
Abstract
- Elevated levels of cadmium (Cd) have the ability to impede plant development. Aldo-keto reductases (AKRs) have been demonstrated in a number of plant species to improve tolerance to a variety of abiotic stresses by scavenging cytotoxic aldehydes; however, only a few AKRs have been identified to improve Cd tolerance. The OsAKR1 gene was extracted and identified from rice here. After being exposed to Cd, the expression of OsAKR1 dramatically rose in both roots and shoots, although more pronounced in roots. According to a subcellular localization experiment, the nucleus and cytoplasm are where OsAKR1 is primarily found. Mutants lacking OsAKR1 exhibited Cd sensitive phenotype than that of the wild-type (WT) Nipponbare (Nip), and osakr1 mutants exhibited reduced capacity to scavenge methylglyoxal (MG). Furthermore, osakr1 mutants exhibited considerably greater hydrogen peroxide (H <subscript>2</subscript> O <subscript>2</subscript> ) and malondialdehyde (MDA) levels, and increased catalase (CAT) activity in comparison to Nip. The expression of three isomeric forms of CAT was found to be considerably elevated in osakr1 mutants during Cd stress, as demonstrated by quantitative real-time PCR analysis, when compared to Nip. These results imply that OsAKR1 controlled rice's ability to withstand Cd by scavenging harmful aldehydes and turning on the reactive oxygen species (ROS) scavenging mechanism.<br />Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.<br /> (Copyright © 2024 Elsevier B.V. All rights reserved.)
- Subjects :
- Aldehydes metabolism
Catalase metabolism
Catalase genetics
Aldehyde Reductase genetics
Aldehyde Reductase metabolism
Malondialdehyde metabolism
Stress, Physiological
Pyruvaldehyde metabolism
Gene Expression Regulation, Plant drug effects
Hydrogen Peroxide metabolism
Plant Proteins genetics
Plant Proteins metabolism
Mutation
Plant Roots metabolism
Plant Roots drug effects
Plant Roots genetics
Inactivation, Metabolic
Oryza genetics
Oryza metabolism
Oryza drug effects
Oryza growth & development
Cadmium toxicity
Cadmium metabolism
Aldo-Keto Reductases genetics
Aldo-Keto Reductases metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1873-3336
- Volume :
- 470
- Database :
- MEDLINE
- Journal :
- Journal of hazardous materials
- Publication Type :
- Academic Journal
- Accession number :
- 38583205
- Full Text :
- https://doi.org/10.1016/j.jhazmat.2024.134212