Asynchronous characteristics of Feammox and iron reduction from paddy soils in Southern China.

Recently, the newly discovered anaerobic ammonium oxidation coupled with iron reduction (i.e., Feammox) has been proven to be a widespread nitrogen (N) loss pathway in ecosystems and has an essential contribution to gaseous N loss in paddy soil. However, the mechanism of iron-nitrogen coupling transformation and the role of iron-reducing bacteria (IRB) in Feammox were poorly understood. This study investigated the Feammox and iron reduction changes and microbial community evolution in a long-term anaerobic incubation by ¹⁵ N isotope labeling combined with molecular biological techniques. The average rates of Feammox and iron reduction during the whole incubation were 0.25 ± 0.04 μg N g ^-1 d ^-1 and 40.58 ± 3.28 μg Fe g ^-1 d ^-1 , respectively. High iron oxide content increased the Feammox rate, but decreased the proportion of Feammox-N ₂ in three Feammox pathways. RBG-13-54-9, Brevundimonas, and Pelomonas played a vital role in the evolution of microbial communities. The characteristics of asynchronous changes between Feammox and iron reduction were found through long-term incubation. IRB might not be the key species directly driving Feammox, and it is necessary to reevaluate the role of IRB in Feammox process.
Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests:Zhengbo Yue reports financial support was provided by National Natural Science Foundation of China. Ding Ma reports financial support was provided by Anhui Provincial Natural Science Foundation. Ding Ma reports financial support was provided by Fundamental Research Funds for the Central Universities. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
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