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In vitro and in silico investigation of glycyrrhizic acid encapsulated zein nanoparticles: A synergistic targeted drug delivery approach for breast cancer.
- Source :
-
International journal of biological macromolecules [Int J Biol Macromol] 2024 May; Vol. 266 (Pt 2), pp. 131368. Date of Electronic Publication: 2024 Apr 03. - Publication Year :
- 2024
-
Abstract
- This study presents an innovative approach for targeted drug delivery through the development of Glycyrrhizic acid-loaded zein nanoparticles (GA-LNPs) as a proficient carrier system. The juxtaposition of zein, a hydrophobic biological macromolecule as a protein carrier, and Glycyrrhizic acid (GA), a hydrophilic therapeutic compound, exemplifies the adaptability of hydrocolloids within cutting-edge drug delivery systems. The characterization and functional traits of research encompass multifaceted analyses of natural macromolecules, which elucidate the homogeneous and spherical morphology of GA-LNPs with an average size of 170.49 nm. The controlled drug release profile of GA, orchestrated under simulated gastrointestinal conditions, adheres to diffusion-based Higuchi kinetics, reflecting the controlled release of the natural macromolecules. The intermolecular interactions among Zein, GA, and cross-linker EDC, facilitated through molecular dynamics simulations, fortify the structural integrity of the encapsulation matrix. In Vitro studies revealed enhanced cellular uptake of GA-LNPs in MCF-7 breast cancer cells. This cellular internalization was further confirmed through cytotoxicity assessments using MTT and apoptosis assays (fluorescence microscopy), which demonstrated the prominent anticancer effects of GA-LNPs on MCF-7 in time/dose-dependent manner. The successful formulation of GA-LNPs, coupled with their sustained release and potent anticancer properties, makes them a potential platform for advanced targeted therapeutic strategies in biomedical applications.<br />Competing Interests: Declaration of competing interest The authors declare that they have no competing interests.<br /> (Copyright © 2024 Elsevier B.V. All rights reserved.)
- Subjects :
- Humans
MCF-7 Cells
Drug Delivery Systems
Drug Liberation
Apoptosis drug effects
Molecular Dynamics Simulation
Female
Antineoplastic Agents pharmacology
Antineoplastic Agents chemistry
Computer Simulation
Cell Survival drug effects
Glycyrrhizic Acid chemistry
Glycyrrhizic Acid pharmacology
Zein chemistry
Nanoparticles chemistry
Breast Neoplasms drug therapy
Breast Neoplasms pathology
Breast Neoplasms metabolism
Drug Carriers chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1879-0003
- Volume :
- 266
- Issue :
- Pt 2
- Database :
- MEDLINE
- Journal :
- International journal of biological macromolecules
- Publication Type :
- Academic Journal
- Accession number :
- 38580025
- Full Text :
- https://doi.org/10.1016/j.ijbiomac.2024.131368