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A novel exoskeletal-derived C-type lectin facilitates phagocytosis of hemocytes in the oriental river prawn Macrobrachium nipponense.
- Source :
-
Fish & shellfish immunology [Fish Shellfish Immunol] 2024 Jun; Vol. 149, pp. 109532. Date of Electronic Publication: 2024 Apr 04. - Publication Year :
- 2024
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Abstract
- C-type lectins (CTLs) execute critical functions in multiple immune responses of crustaceans as a member of pattern recognition receptors (PRRs) family. In this study, a novel CTL was identified from the exoskeleton of the oriental river prawn Macrobrachium nipponense (MnLec3). The full-length cDNA of MnLec3 was 1150 bp with an open reading frame of 723 bp, encoding 240 amino acids. MnLec3 protein contained a signal peptide and one single carbohydrate-recognition domain (CRD). MnLec3 transcripts were widely distributed at the exoskeleton all over the body. Significant up-regulation of MnLec3 in exoskeleton after Aeromonas hydrophila challenged suggested the involvement of MnLec3 as well as the possible function of the exoskeleton in immune response. In vitro tests with recombinant MnLec3 protein (rMnLec3) manifested that it had polysaccharide binding activity, a wide spectrum of bacterial binding activity and agglutination activity only for tested Gram-negative bacteria (Escherichia coli, Vibrio anguillarum and A. hydrophila). Moreover, rMnLec3 significantly promoted phagocytic ability of hemocytes against A. hydrophila in vivo. What's more, MnLec3 interference remarkably impaired the survivability of the prawns when infected with A. hydrophila. Collectively, these results ascertained that MnLec3 derived from exoskeleton took an essential part in immune defense of the prawns against invading bacteria as a PRR.<br />Competing Interests: Declaration of competing interest The authors declare no conflict of interest.<br /> (Copyright © 2024 Elsevier Ltd. All rights reserved.)
- Subjects :
- Animals
Gene Expression Profiling veterinary
Base Sequence
Animal Shells immunology
Animal Shells chemistry
Palaemonidae immunology
Palaemonidae genetics
Lectins, C-Type genetics
Lectins, C-Type immunology
Lectins, C-Type chemistry
Phagocytosis
Arthropod Proteins genetics
Arthropod Proteins immunology
Arthropod Proteins chemistry
Hemocytes immunology
Immunity, Innate genetics
Amino Acid Sequence
Aeromonas hydrophila physiology
Phylogeny
Sequence Alignment veterinary
Gene Expression Regulation immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1095-9947
- Volume :
- 149
- Database :
- MEDLINE
- Journal :
- Fish & shellfish immunology
- Publication Type :
- Academic Journal
- Accession number :
- 38579977
- Full Text :
- https://doi.org/10.1016/j.fsi.2024.109532