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Hippuric acid alleviates dextran sulfate sodium-induced colitis via suppressing inflammatory activity and modulating gut microbiota.
- Source :
-
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2024 May 28; Vol. 710, pp. 149879. Date of Electronic Publication: 2024 Apr 03. - Publication Year :
- 2024
-
Abstract
- Inflammatory bowel disease (IBD) is a chronic inflammatory disease associated with metabolic disorder and gut dysbiosis. Decreased abundance of hippuric acid (HA) was found in patients with IBD. HA, metabolized directly from benzoic acid in the intestine and indirectly from polyphenols, serves as a marker of polyphenol catabolism. While polyphenols and benzoic acid have been shown to alleviate intestinal inflammation, the role of HA in this context remains unknown. Herein, we investigated the effects and mechanism of HA on DSS-induced colitis mice. The results revealed that HA alleviated clinical activity and intestinal barrier damage, decreased pro-inflammatory cytokine production. Metagenomic sequencing suggested that HA treatment restored the gut microbiota, including an increase in beneficial gut bacteria such as Adlercreutzia, Eubacterium, Schaedlerella and Bifidobacterium&#95;pseudolongum. Furthermore, we identified 113 candidate genes associated with IBD that are potentially under HA regulation through network pharmacological analyses. 10 hub genes including ALB, IL-6, HSP90AA1, and others were identified using PPI analysis and validated using molecular docking and mRNA expression analysis. Additionally, KEGG analysis suggested that the renin-angiotensin system (RAS), NF-κB signaling and Rap1 signaling pathways were important pathways in the response of HA to colitis. Thus, HA may provide novel biotherapy options for IBD.<br />Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Zhenyu Yang reports financial support was provided by Hunan Provincial Natural Science Foundation. Xiaoyan Wang reports financial support was provided by Hunan Provincial Natural Science Foundation. Zhenyu Yang reports financial support was provided by Hunan Provincial Health Commission. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.<br /> (Copyright © 2024 Elsevier Inc. All rights reserved.)
- Subjects :
- Humans
Animals
Mice
Dextran Sulfate
Molecular Docking Simulation
Benzoic Acid
Disease Models, Animal
Mice, Inbred C57BL
Colon
Gastrointestinal Microbiome
Colitis chemically induced
Colitis drug therapy
Inflammatory Bowel Diseases chemically induced
Inflammatory Bowel Diseases drug therapy
Hippurates
Subjects
Details
- Language :
- English
- ISSN :
- 1090-2104
- Volume :
- 710
- Database :
- MEDLINE
- Journal :
- Biochemical and biophysical research communications
- Publication Type :
- Academic Journal
- Accession number :
- 38579536
- Full Text :
- https://doi.org/10.1016/j.bbrc.2024.149879