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Protocol for isolating small cytosolic dsDNA from cultured murine cells.

Authors :
Dai Z
Ji H
Zheng A
Huang AL
Tang KF
Source :
STAR protocols [STAR Protoc] 2024 Jun 21; Vol. 5 (2), pp. 102998. Date of Electronic Publication: 2024 Apr 03.
Publication Year :
2024

Abstract

We recently identified a class of small cytosolic double-stranded DNA (scDNA) approximately 20-40 bp in size in human and mouse cells. Here, we present a protocol for scDNA isolation from cultured murine cells. We describe steps for cytosolic compartment separation, DNA isolation in the cytosolic fraction using phenol-chloroform extraction, and ethanol precipitation. We then detail procedures for denaturing purified cytosolic DNA through urea polyacrylamide gel electrophoresis and obtaining scDNA in the cytosolic DNA fraction via gel purification. For complete details on the use and execution of this protocol, please refer to Liu et al. <superscript>1</superscript> .<br />Competing Interests: Declaration of interests The authors declare no competing interests.<br /> (Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.)

Details

Language :
English
ISSN :
2666-1667
Volume :
5
Issue :
2
Database :
MEDLINE
Journal :
STAR protocols
Publication Type :
Academic Journal
Accession number :
38573862
Full Text :
https://doi.org/10.1016/j.xpro.2024.102998