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Staphylococcus aureus NAD kinase is required for envelop and antibiotic stress responses.
- Source :
-
Microbes and infection [Microbes Infect] 2024 May-Jun; Vol. 26 (4), pp. 105334. Date of Electronic Publication: 2024 Mar 30. - Publication Year :
- 2024
-
Abstract
- Global burden of infectious diseases and antimicrobial resistance are major public health issues calling for innovative control measures. Bacterial NAD kinase (NADK) is a crucial enzyme for production of NADP(H) and growth. In Staphylococcus aureus, NADK promotes pathogenesis by supporting production of key virulence determinants. Here, we find that knockdown of NADK by CRISPR interference sensitizes S. aureus to osmotic stress and to stresses induced by antibiotics targeting the envelop as well as replication, transcription and translation. Thus, NADK represents a promising target for the development of inhibitors which could be used in combination with current antibiotics.<br />Competing Interests: Declaration of competing interest The authors declare that they have no conflict of interests.<br /> (Copyright © 2024 The Authors. Published by Elsevier Masson SAS.. All rights reserved.)
- Subjects :
- Bacterial Proteins metabolism
Bacterial Proteins genetics
Gene Expression Regulation, Bacterial
Gene Knockdown Techniques
Osmotic Pressure
Stress, Physiological
Anti-Bacterial Agents pharmacology
Phosphotransferases (Alcohol Group Acceptor) metabolism
Phosphotransferases (Alcohol Group Acceptor) genetics
Staphylococcus aureus enzymology
Staphylococcus aureus genetics
Staphylococcus aureus drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1769-714X
- Volume :
- 26
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Microbes and infection
- Publication Type :
- Academic Journal
- Accession number :
- 38556158
- Full Text :
- https://doi.org/10.1016/j.micinf.2024.105334