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Genetic characterization of Toxoplasma gondii strains isolated from humans living in İzmir, Türkiye.
- Source :
-
Indian journal of medical microbiology [Indian J Med Microbiol] 2024 May-Jun; Vol. 49, pp. 100571. Date of Electronic Publication: 2024 Apr 04. - Publication Year :
- 2024
-
Abstract
- Purpose: Toxoplasma gondii is an obligate intracellular zoonotic parasite that can infect all warm-blooded animals, including humans. Currently, clinical findings of toxoplasmosis are being related to T. gondii strains such as Type I genotype may cause high pathogenicity and Type II genotype causes a milder clinical presentation. We have showed in our previous that Type II genotype is the most frequent strain detected in stray cats and wild birds living in natural life of İzmir. The aim of this study was to assess toxoplasmosis seroprevalence in immunocompromised patients, investigate the presence of T. gondii DNA in their blood samples, and genotype the PCR positive ones.<br />Methods: The 42 buffy-coat and serum samples were collected from immunocompromised patients who were from various clinics. Thereafter, Real-Time PCR targeting RE gene of T. gondii was performed with DNA samples obtained from buffy-coat samples. Genotyping was performed by sequencing of GRA6 and GRA7 gene regions of positive DNA samples obtained from tissues of bioassay and PCR positive samples.<br />Results: According to Real-Time PCR results, T. gondii DNA was detected in 23.8% (10/42) samples. Among these 10 samples, two samples were determined as T. gondii Type II genotype. Anti-Toxoplasma IgG antibodies were detected in 28.57% (12/42) samples.<br />Conclusions: Overall, the detection of Type II genotype in humans in İzmir province suggested that T. gondii infection in humans, stray cats, and wild animals may be associated to each other in terms of transmission.<br />Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.<br /> (Copyright © 2024 Indian Association of Medical Microbiologists. Published by Elsevier B.V. All rights reserved.)
- Subjects :
- Humans
Male
Female
Adult
Middle Aged
Protozoan Proteins genetics
Seroepidemiologic Studies
Real-Time Polymerase Chain Reaction
Immunocompromised Host
Animals
Antigens, Protozoan genetics
Antibodies, Protozoan blood
Young Adult
Cats
Adolescent
Aged
Child
Toxoplasma genetics
Toxoplasma isolation & purification
Toxoplasma immunology
Toxoplasma classification
Toxoplasmosis parasitology
Toxoplasmosis epidemiology
Genotype
DNA, Protozoan genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1998-3646
- Volume :
- 49
- Database :
- MEDLINE
- Journal :
- Indian journal of medical microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 38554777
- Full Text :
- https://doi.org/10.1016/j.ijmmb.2024.100571