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Explore the active ingredients and potential mechanism of action on Actinidia arguta leaves against T2DM by integration of serum pharmacochemistry and network pharmacology.
- Source :
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Journal of pharmaceutical and biomedical analysis [J Pharm Biomed Anal] 2024 Jul 15; Vol. 244, pp. 116105. Date of Electronic Publication: 2024 Mar 13. - Publication Year :
- 2024
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Abstract
- Background: Actinidia arguta leaves (AAL) are traditionally consumed as a vegetable and as tea in folk China and Korea. Previous studies have reported the anti-diabetic effect of AAL, but its bioactive components and mechanism of action are still unclear.<br />Aim of the Study: This study aims to identify the hypoglycemic active components of AAL by combining serum pharmacochemistry and network pharmacology and to elucidate its possible mechanism of action.<br />Methods: Firstly, the effective components in mice serum samples were characterized by UPLC-Q/TOF-MS <superscript>E</superscript> . Furthermore, based on these active ingredients, network pharmacology analysis was performed to establish an "H-C-T-P-D" interaction network and reveal possible biological mechanisms. Finally, the affinity between serum AAL components and the main proteins in the important pathways above was investigated through molecular docking analysis.<br />Results: Serum pharmacochemistry analysis showed that 69 compounds in the serum samples were identified, including 23 prototypes and 46 metabolites. The metabolic reactions mainly included deglycosylation, dehydration, hydrogenation, methylation, acetylation, glucuronidation, and sulfation. Network pharmacology analysis showed that the key components quercetin, pinoresinol diglucoside, and 5-O-trans-p-coumaroyl quinic acid butyl ester mainly acted on the core targets PTGS2, HRAS, RELA, PRKCA, and BCL2 targets and through the PI3K-Akt signaling pathway, endocrine resistance, and MAPK signaling pathway to exert a hypoglycemic effect. Likewise, molecular docking results showed that the three potential active ingredients had good binding effects on the five key targets.<br />Conclusion: This study provides a basis for elucidating the pharmacodynamic substance basis of AA against T2DM and further exploring the mechanism of action.<br />Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.<br /> (Copyright © 2024. Published by Elsevier B.V.)
- Subjects :
- Animals
Mice
Male
Chromatography, High Pressure Liquid methods
Signal Transduction drug effects
Actinidia chemistry
Plant Leaves chemistry
Network Pharmacology
Molecular Docking Simulation
Hypoglycemic Agents pharmacology
Hypoglycemic Agents chemistry
Plant Extracts pharmacology
Plant Extracts chemistry
Diabetes Mellitus, Type 2 drug therapy
Diabetes Mellitus, Type 2 blood
Subjects
Details
- Language :
- English
- ISSN :
- 1873-264X
- Volume :
- 244
- Database :
- MEDLINE
- Journal :
- Journal of pharmaceutical and biomedical analysis
- Publication Type :
- Academic Journal
- Accession number :
- 38552420
- Full Text :
- https://doi.org/10.1016/j.jpba.2024.116105