Identification and Molecular Characterization of Giant Liver Fluke ( Fascioloides magna ) Infection in European Fallow Deer ( Dama dama ) in Romania-First Report.

Fascioloidosis is a parasitic disease of primary wild and domestic ruminants, caused by giant liver fluke, Fascioloides magna . The definitive host of the liver fluke in its area of origin (North America) is the white-tailed deer ( Odocoileus virginianus ). In Europe, the red deer ( Cervus elaphus ) and European fallow deer ( Dama dama ) are definitive hosts and the most sensitive hosts to F. magna infection, on which the parasite exerts serious pathogenic effects. In this study, we analyzed fecal samples and livers of 72 D. dama from 11 hunting grounds in Arad County, Romania. Of the 72 fecal samples and livers from D. dama , trematodes of the genus Fascioloides were identified in four (5.56%). Sequencing revealed that the trematodes identified in the samples were similar to the sequence of F. magna (GenBank no. EF534992.1, DQ683545.1, KU232369.1). The sequence obtained from the molecular analysis has been deposited in GenBank ^® under accession number OQ689976.1. This study describes the first report of giant liver fluke ( F. magna ) infection in D. dama in Romania.